SMPDL3A gene is a novel liver X receptor (LXR) -regulated gene, with an LXR response element within its promoter. The induction of SMPDL3A is LXR-dependent and is restricted to human blood cells with no induction observed in mouse cellular systems. LXR ? and LXRβ function as physiological sensors of cholesterol metabolites (oxysterols), regulating key genes involved in cholesterol and lipid metabolism. LXRs have been extensively studied in both human and rodent cell systems, revealing their potential therapeutic value in the contexts of atherosclerosis and inflammatory diseases. The LXR genome landscape has been investigated in murine macrophages but not in human THP-1 cells, which represent one of the frequently used monocyte/macrophage cell systems to study immune responses.
Product Name:
Mouse SMPDL3A Recombinant Protein (RPES2204)
Product Code:
RPES2204
Size:
20µg
Species:
Mouse
Expressed Host:
HEK293 Cells
Synonyms:
0610010C24Rik,AI529588,ASM3A,ASML3,ASML3A
Accession:
NP_065586.3
Sequence:
Met1-Leu445
Fusion tag:
C-His
Endotoxin:
<1.0 EU per µg of the protein as determined by the LAL method.
Protein Construction:
A DNA sequence encoding the mouse Smpdl3a (NP_065586.3) (Met1-Leu445) was expressed with a polyhistidine tag at the C-terminus.
Purity:
> 95 % as determined by SDS-PAGE
Mol Mass:
49.1 kDa
Formulation:
Lyophilized from sterile PBS, pH 7.4
Shipping:
This product is provided as lyophilized powder which is shipped with ice packs.
Stability and Storage:
Lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
