Recombinant Mouse B7-H2/ICOSLG/CD275 Protein (RPCB1273)
- SKU:
- RPCB1273
- Size:
- 10µg
- Tag:
- C-His
- Reactivity:
- Mouse
- Expression Host:
- HEK293 cells
Description
| Product Name: | Recombinant Mouse B7-H2/ICOSLG/CD275 Protein |
| SKU: | RPCB1273 |
| Size: | 10µg |
| Tag: | C-His |
| Reactivity: | Mouse |
| Expression Host: | HEK293 cells |
| Protein Description: | High quality, high purity and low endotoxin recombinant Recombinant Mouse B7-H2/ICOSLG/CD275 Protein , tested reactivity in HEK293 cells and has been validated in SDS-PAGE.100% guaranteed. |
| Endotoxin: | <0.1EU/μg |
| Purity: | > 95% by SDS-PAGE. |
| Formulation: | Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4. |
| Gene ID: | 50723 |
Inducible co-stimulator ligand (ICOSL), also known as B7-H2, is a member of the B7 family of co-stimulatory molecules related to B7-1 and B7-2. It is a transmembrane glycoprotein with extracellular IgV and IgC domains and binds to ICOS on activated T cells, thus delivers a positive costimulatory signal for optimal T cell function. The structural features of ICOSL are crucial for its costimulatory function. The present study shows that ICOSL displays a marked oligomerization potential, resembling more like B7-1 than B7-2. B7-H2-dependent signaling may play an active role in a proliferative response rather than in cytokine and chemokine production. The CD28/B7 and ICOS/B7-H2 pathways are both critical for costimulating T cell immune responses. Deficiency in either pathway results in defective T cell activation, cytokine production, and germinal center formation.
| Storage: | Store at -20℃.Store the lyophilized protein at -20℃ to -80 ℃ up to 1 year from the date of receipt.After reconstitution, the protein solution is stable at -20℃ for 3 months, at 2-8℃ for up to 1 week. |
| Reconstitution: | Centrifuge the vial before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid vortex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles. |
| Swiss-Prot: | Q9JHJ8 |
 | Recombinant Mouse B7- H2/ICOSLG/CD275 Protein was determined by SDS-PAGE with Coomassie Blue, showing a band at 38-50kDa. |
 | Immobilized Mouse ICOSLG at 5 μg/mL (100 μL/well) can bind Mouse ICOS with a linear range of 10-952.7 ng/mL . |
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