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Recombinant Human CD3E&CD3D Protein (RPCB0693)

SKU:
RPCB0693
Size:
100µg
Tag:
C-His
Reactivity:
Human
Expression Host:
E. coli
€669
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Description

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Product Name:Recombinant Human CD3E&CD3D Protein
SKU:RPCB0693
Size:100µg
Tag:C-His
Reactivity:Human
Expression Host:E. coli
Protein Description:High quality, high purity and low endotoxin recombinant Recombinant Human CD3E&CD3D Protein , tested reactivity in HEK293 cells and has been validated in SDS-PAGE.100% guaranteed.
Endotoxin:< 1 EU/μg of the protein by LAL method
Purity:> 95% by SDS-PAGEï¼›> 95% by HPLC
Formulation:Lyophilized from 0.22 μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Gene ID:916&915

T-cell surface glycoprotein CD3 epsilon & CD3 delta chain, also known as CD3E & CD3D , are single-pass type I membrane proteins.When antigen presenting cells (APCs) activate T-cell receptor (TCR), TCR-mediated signals are transmitted across the cell membrane by the CD3 chains CD3D, CD3E, CD3G and CD3Z. All CD3 chains contain immunoreceptor tyrosine-based activation motifs (ITAMs) in their cytoplasmic domain.

Storage:Store at -20℃.Store the lyophilized protein at -20℃ to -80 ℃ up to 1 year from the date of receipt.After reconstitution, the protein solution is stable at -20℃ for 3 months, at 2-8℃ for up to 1 week.
Reconstitution:Centrifuge the vial before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid vortex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles.
Swiss-Prot:P07766(CD3E)&P04234(CD3D)
Product ImageHuman CD3E&CD3D on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.
Product ImageThe purity of Human CD3E&CD3D was greater than 95% as determined by SEC-HPLC.
Product ImageHuman CD3E&CD3D, His Tag captured on CM5 Chip via Anti-His Antibody can bind OKT3, mFc Tag with an affinity constant of 0.36nM as determined in SPR assay (Biacore T200).

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