null

Rat Lysyl Oxidase Like Protein 2 (LOXL2) ELISA Kit

SKU:
RTDL00638
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
B5DF27
Sensitivity:
0.057ng/mL
Range:
0.156-10ng/mL
ELISA Type:
Sandwich
Synonyms:
LOR2,WS9-14,Lysyl oxidase homolog 2,Lysyl oxidase-related protein 2,Lysyl oxidase-related protein WS9-14
Reactivity:
Rat
Sample Type:
Serum, Plasma, Tissue Homogenates And Other Biological Fluids
€749
Frequently bought together:

Description

system_update_altDatasheet

Rat Lysyl Oxidase Like Protein 2 (LOXL2) ELISA Kit

The Rat Lysyl Oxidase-Like Protein 2 (LOXL2) ELISA Kit is a powerful tool for the quantitative measurement of LOXL2 levels in rat samples, including serum, plasma, and tissue homogenates. With its high sensitivity and specificity, this kit provides accurate and reliable results for your research needs.LOXL2 is an important enzyme that plays a key role in extracellular matrix remodeling and fibrosis. Dysregulation of LOXL2 has been implicated in various pathologies, including cancer, fibrosis, and tissue stiffness.

By measuring LOXL2 levels, researchers can gain valuable insights into disease mechanisms and potential therapeutic targets.This ELISA kit offers a simple and efficient assay format, making it suitable for high-throughput analysis. With its robust performance and versatility, the Rat LOXL2 ELISA Kit is an indispensable tool for investigating the role of LOXL2 in disease pathology and developing novel treatment strategies.

Product Name:Rat Lysyl Oxidase Like Protein 2 (LOXL2) ELISA Kit
Product Code:RTDL00638
Size:96 Assays
Target:LOXL2
Synonyms:LOR2, WS9-14, Lysyl oxidase homolog 2, Lysyl oxidase-related protein 2, Lysyl oxidase-related protein WS9-14
Detection Method:Sandwich
Range:0.156-10ng/mL
Shelf Life:12 months
Note:For research use only
Recovery:Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
MatrixRecovery range (%)Average(%)
Serum (n=5)80-10291
EDTA plasma (n=5)81-10090
Heparin plasma (n=5)80-8984
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
Serum (n=5) 82-96% 83-98% 81-99% 93-101%
EDTA plasma (n=5) 88-101% 86-95% 90-102% 80-93%
Heparin plasma (n=5) 80-91% 82-90% 95-104% 79-95%
Intra-assay Precision:Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
StepProtocol
1. Prepare all reagents, samples and standards
2.Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.Aspirate and wash 3 times
5.Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.Aspirate and wash 5 times
7.Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.Add 50µL Stop Solution. Read at 450nm immediately.

Related Products