The Rat FST (Follistatin) ELISA Kit is a specialized assay designed for the precise and quantitative measurement of Follistatin levels in rat biological samples. Follistatin is a crucial regulatory protein that plays a key role in modulating the bioactivity of members of the TGF-β superfamily, such as activin, BMPs, and myostatin. Follistatin is involved in various biological processes, including cell growth, differentiation, and tissue homeostasis. Accurate quantification of Follistatin levels using this ELISA kit is essential for investigating the regulatory functions of Follistatin in different physiological and pathological conditions.
Follistatin is known to be involved in developmental processes, muscle growth, inflammation, and reproductive functions, making it a significant target for research in fields such as developmental biology, endocrinology, and muscle physiology. The Rat FST ELISA Kit from Assay Genie offers exceptional sensitivity and specificity, ensuring accurate and reproducible results. Manufactured under stringent quality control standards, this kit provides reliable performance and ease of use, making it an excellent tool for researchers studying the multifaceted roles of Follistatin in various biological systems.
Product Name:
Rat FST (Follistatin) ELISA Kit
SKU:
AEES00399
Target:
Rat FST (Follistatin)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
0.10 ng/mL
Detection range:
0.16-10 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Rat FST. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat FST and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat FST, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Rat FST. You can calculate the concentration of Rat FST in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
90-104
88-103
91-108
Average (%)
95
96
98
1:4
Range (%)
91-102
80-91
85-98
Average (%)
97
86
91
1:8
Range (%)
92-102
83-96
82-93
Average (%)
97
90
88
1:16
Range (%)
91-101
82-92
88-103
Average (%)
96
87
94
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
90-105
97
EDTA plasma (n=5)
86-97
92
Cell culture media (n=5)
83-98
90
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
0.5
1.26
3.74
0.5
1.35
4.04
Standard deviation
0.03
0.05
0.12
0.03
0.08
0.22
C V (%)
6
3.97
3.21
6.0
5.93
5.45
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Rat FST concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Rat FST in samples. No significant cross-reactivity or interference between Rat FST and analogues was observed.
