The Rat AChE (Acetylcholinesterase) ELISA Kit is specifically designed for the quantitative analysis of acetylcholinesterase levels in various rat biological samples. Acetylcholinesterase is a vital enzyme involved in the hydrolysis of the neurotransmitter acetylcholine, thereby contributing to the termination of synaptic transmission at cholinergic synapses. The precise regulation of acetylcholinesterase activity is crucial for maintaining proper neuronal signaling, neuromuscular function, and cognitive processes. In various tissues, particularly in the nervous system, acetylcholinesterase activity is tightly controlled to ensure accurate neurotransmission and prevent the accumulation of acetylcholine.
Dysregulation of AChE levels or activity can lead to neurological disorders, cognitive impairments, and neuromuscular dysfunction. The Rat AChE ELISA Kit from Assay Genie provides researchers with a reliable and accurate tool to measure acetylcholinesterase levels with exceptional sensitivity and specificity. This kit is manufactured under rigorous quality control standards to ensure dependable performance and reproducible results, making it an excellent choice for studies focusing on cholinergic neurotransmission, neurodegenerative diseases, and neurobiology research.
Product Name:
Rat AChE (Acetylcholinesterase) ELISA Kit
SKU:
AEES00387
Target:
Rat AChE (Acetylcholinesterase)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
0.47 ng/mL
Detection range:
0.78-50 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Rat AChE. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat AChE and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat AChE, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Rat AChE. You can calculate the concentration of Rat AChE in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
90-100
91-107
98-110
Average (%)
95
99
103
1:4
Range (%)
93-107
85-97
86-99
Average (%)
100
92
93
1:8
Range (%)
88-103
87-100
89-99
Average (%)
96
92
94
1:16
Range (%)
88-101
88-100
89-100
Average (%)
94
93
95
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
92-103
97
EDTA plasma (n=5)
89-99
94
Cell culture media (n=5)
90-106
97
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
2.43
7.13
17.7
2.43
7.84
16.22
Standard deviation
0.13
0.29
0.91
0.13
0.42
0.57
C V (%)
5.35
4.07
5.14
5.35
5.36
3.51
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Rat AChE concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Rat AChE in samples. No significant cross-reactivity or interference between Rat AChE and analogues was observed.
