The QuickStep Canine PG (Progesterone) ELISA Kit is specifically designed for the precise measurement of progesterone levels in canine serum, plasma, and other biological samples. With its high sensitivity and specificity, this kit provides dependable and consistent results, making it an invaluable tool for researchers and veterinarians working in the field of canine reproduction.Progesterone is a hormone essential for the regulation of the estrous cycle in female dogs, as well as for maintaining pregnancy. Monitoring progesterone levels is critical for determining the optimal time for breeding, confirming ovulation, and predicting whelping dates.
This kit allows for easy and accurate quantification of progesterone, aiding in the management of breeding programs and ensuring the health and well-being of canine patients.Whether studying reproductive physiology, breeding management, or assessing hormonal imbalances in dogs, the QuickStep Canine PG ELISA Kit offers a reliable and convenient solution for measuring progesterone levels in canine samples. Trust in this kit for accurate results and valuable insights into canine reproductive health.
Product Name:
QuickStep Canine Progesterone ELISA Kit
Product Code:
QSES009
Assay Type:
Competitive
Assay Format:
96T
Assay Time:
1.5h
Reactivity:
Canine
Sensitivity:
6.58 pg/mL
Range:
15.63-1000 pg/mL
Detection Method:
Colormetric
Sample Type:
Serum and plasma
Target:
Progesterone
Application:
This ELISA kit applies to the in vitro quantitative determination of Pg concentrations in serum and plasma.
Specificity:
This kit recognizes Pg in samples. No significant cross-reactivity or interference between Pg and analogues was observed.
Dilution Method:
It is recommended to dilute normal serum/plasma samples at 10 fold.
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Storage & Stability:
An unopened kit can be stored at 2-8°C for 6 months. After test, the unused wells and reagents should be stored according to the storage table in the manual.
This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with Canine Pg. Samples (or Standards) and Horseradish Peroxidase (HRP) linked antibody specific for Canine Pg are added to the micro ELISA plate wells. Canine Pg in samples (or standards) competes with a fixed amount of Canine Pg on the solid phase supporter for sites on the HRP linked detection antibody specific to Canine Pg. Excess conjugate and unbound sample or standard are washed from the plate. The substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 ± 2 nm. The concentration of Canine Pg in the samples is then determined by comparing the OD of the samples to the standard curve.
Item
Specifications
Storage
Micro ELISA Plate(Dismountable)
8 wells - 12 strips
2-8°C, 1 months
Reference Standard
2 vials
2-8°C, use the reconstituted standard within 24h
Concentrated HRP Linked Detection Ab(100-)
1 vial, 60 µL
2-8°C(Protect from light)
Reference Standard & Sample Diluent
1 vial, 20 mL
2-8°C
HRP Linked Ab Diluent
1 vial, 14 mL
Concentrated Wash Buffer(25-)
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
2-8°C(Protect from light)
Stop Solution
1 vial, 10 mL
2-8°C
Plate Sealer
5 pieces
Product Description
1 copy
Certificate of Analysis
1 copy
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20
20
20
20
20
Mean (pg/mL)
51.75
117.46
374.14
54.00
120.75
362.03
Standard deviation
3.32
6.11
19.53
2.70
4.94
16.62
CV (%)
6.42
5.20
5.22
5.00
4.09
4.59
Sample Type
Range (%)
Average Recovery (%)
Serum(n=8)
90-100
95
EDTA plasma(n=8)
94-108
100
Samples were spiked with high concentrations of Canine Pg and diluted with Reference Standard & Sample Diluent to produceSamples with values within the range of the assay.
