PSAP Polyclonal Antibody (CAB21761)
- SKU:
- CAB21761
- Product Type:
- Antibody
- Antibody Type:
- Polyclonal Antibody
- Reactivity:
- Human
- Mouse
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Applications:
- WB
- IHC
- IF
Description
| Product Name: | PSAP Polyclonal Antibody |
| SKU: | CAB21761 |
| Size: | 20uL, 100uL |
| Isotype: | IgG |
| Host Species: | Rabbit |
| Reactivity: | Human,Mouse,Rat |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 275-524 of human PSAP (NP_002769.1). |
| Sequence: | KEMP MQTL VPAK VASK NVIP ALEL VEPI KKHE VPAK SDVY CEVC EFLV KEVT KLID NNKT EKEI LDAF DKMC SKLP KSLS EECQ EVVD TYGS SILS ILLE EVSP ELVC SMLH LCSG TRLP ALTV HVTQ PKDG GFCE VCKK LVGY LDRN LEKN STKQ EILA ALEK GCSF LPDP YQKQ CDQF VAEY EPVL IEIL VEVM DPSF VCLK IGAC PSAH KPLL GTEK CIWG PSYW CQNT ETAA QCNA VEHC KRHV WN |
| Tested Applications: | WB IHC-P IF/ICC ELISA |
| Recommended Dilution: | WB,1:500 - 1:1000 IHC-P,1:50 - 1:200 IF/ICC,1:50 - 1:200 |
| Synonyms: | GLBA; SAP1; SAP2; PSAPD; PARK24; AP |
| Positive Sample: | 293T,HepG2,A-431,Mouse brain,Rat brain |
| Conjugate: | Unconjugated |
| Cellular Localization: | Lysosome, Secreted. |
| Calculated MW: | 58kDa |
| Observed MW: | 63kDa |
This gene encodes a highly conserved preproprotein that is proteolytically processed to generate four main cleavage products including saposins A, B, C, and D. Each domain of the precursor protein is approximately 80 amino acid residues long with nearly identical placement of cysteine residues and glycosylation sites. Saposins A-D localize primarily to the lysosomal compartment where they facilitate the catabolism of glycosphingolipids with short oligosaccharide groups. The precursor protein exists both as a secretory protein and as an integral membrane protein and has neurotrophic activities. Mutations in this gene have been associated with Gaucher disease and metachromatic leukodystrophy. Alternative splicing results in multiple transcript variants, at least one of which encodes an isoform that is proteolytically processed.
| Purification Method: | Affinity purification |
| Gene ID: | 5660 |
| Storage Buffer: | Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.05% proclin300,50% glycerol,pH7.3. |
 | Western blot analysis of various lysates, using [KO Validated] PSAP Rabbit pAb (CAB21761) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 30s. |
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