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Porcine Osteopontin (OPN) ELISA Kit

SKU:
PRDL00122
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
P14287
Sensitivity:
29.8pg/mL
Range:
62.5-4000pg/mL
ELISA Type:
Sandwich
Synonyms:
SPP1,BNSP,BSPI,ETA1,Secreted Phosphoprotein 1,Bone Sialoprotein I,Early T-Lymphocyte Activation 1,Nephropontin,Urinary stone protein,Uropontin
Reactivity:
Porcine
Sample Type:
Serum, Plasma, Tissue Homogenates, Cell Lysates And Other Biological Fluids
€749
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Description

system_update_altDatasheet

Porcine Osteopontin (OPN) ELISA Kit

The Porcine Osteopontin (OPN) ELISA Kit is specially designed for the accurate detection of osteopontin levels in porcine serum, plasma, and cell culture supernatants. This kit boasts high sensitivity and specificity, ensuring dependable and reproducible results for various research applications.Osteopontin is a key protein involved in bone metabolism, inflammation, and tissue remodeling. It plays a critical role in conditions such as osteoporosis, arthritis, and cancer, making it an important biomarker for studying these diseases and potential therapeutic interventions.

With the Porcine Osteopontin ELISA Kit, researchers can confidently measure osteopontin levels in porcine samples, providing valuable insights into these physiological processes and diseases. This kit is an essential tool for advancing research in the fields of bone biology, immunology, and oncology.

Product Name:Porcine Osteopontin (OPN) ELISA Kit
Product Code:PRDL00122
Size:96 Assays
Target:OPN
Synonyms:SPP1, BNSP, BSPI, ETA1, Secreted Phosphoprotein 1, Bone Sialoprotein I, Early T-Lymphocyte Activation 1, Nephropontin, Urinary stone protein, Uropontin
Detection Method:Sandwich
Range:62.5-4,000pg/mL
Shelf Life:12 months
Note:For research use only
Recovery:Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
MatrixRecovery range (%)Average(%)
Serum (n=5)80-10291
EDTA plasma (n=5)81-9990
Heparin plasma (n=5)80-8984
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
Serum (n=5) 82-96% 83-98% 81-99% 93-101%
EDTA plasma (n=5) 88-101% 86-95% 90-102% 80-93%
Heparin plasma (n=5) 80-91% 82-90% 95-104% 79-95%
Intra-assay Precision:Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
StepProtocol
1. Prepare all reagents, samples and standards
2.Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.Aspirate and wash 3 times
5.Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.Aspirate and wash 5 times
7.Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.Add 50µL Stop Solution. Read at 450nm immediately.

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