The PITX3 Polyclonal Antibody (CAB10569) is a vital tool for researchers studying the PITX3 protein, a transcription factor involved in the development and maintenance of dopaminergic neurons in the brain. This antibody, produced in rabbits, is highly specific to human samples and has been validated for use in Western blot applications.PITX3 is known for its role in regulating the expression of genes involved in dopamine production, making it an important target for research in neuroscience and neurodegenerative diseases such as Parkinson's disease.
By detecting and analyzing PITX3 protein levels in various cell types, this antibody provides valuable insight into the mechanisms underlying dopaminergic neuron function and dysfunction.Understanding the function of PITX3 is essential for advancing our knowledge of the brain's dopaminergic system and developing potential therapies for neurodegenerative disorders. The PITX3 Polyclonal Antibody offers researchers a reliable tool for investigating the role of PITX3 in normal brain function and disease pathology.
Product Name:
PITX3 Rabbit Polyclonal Antibody
SKU:
CAB10569
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-70 of human PITX3 (NP_005020.1).
Sequence:
MEFG LLSE AEAR SPAL SLSD AGTP HPQL PEHG CKGQ EHSD SEKA SASL PGGS PEDG SLKK KQRR QRTH FT
Tested Applications:
WBELISA
Recommended Dilution:
WB,1:500 - 1:2000
Synonyms:
ASMD; ASOD; PTX3; ASGD1; CTPP4; CTRCT11; PITX3
Positive Sample:
293T,U-87MG,Mouse heart,Rat kidney,Rat brain
Conjugate:
Unconjugated
Cellular Localization:
Nucleus.
Calculated MW:
32kDa
Observed MW:
32kDa
This gene encodes a member of the RIEG/PITX homeobox family, which is in the bicoid class of homeodomain proteins. Members of this family act as transcription factors. This protein is involved in lens formation during eye development. Mutations of this gene have been associated with anterior segment mesenchymal dysgenesis and congenital cataracts.
Purification Method:
Affinity purification
Gene ID:
5309
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Western blot analysis of extracts of various cell lines, using PITX3 antibody (CAB10569) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 10s.
