The Phospho-Smad2 (S465/467) / Smad3 (S423/425) Polyclonal Antibody (CABP0548) is a valuable tool for researchers studying the Smad2 and Smad3 proteins, key components of the TGF-beta signaling pathway. This antibody, raised in rabbits, is highly specific for phosphorylated Smad2 at serine 465/467 and phosphorylated Smad3 at serine 423/425 in human samples, allowing for accurate detection and analysis in Western blot applications. The TGF-beta signaling pathway is involved in numerous cellular processes, including cell growth, differentiation, and apoptosis. Dysregulation of this pathway has been implicated in various diseases, including cancer, fibrosis, and immune disorders.
The ability to specifically detect and study the phosphorylation status of Smad2 and Smad3 provides valuable insights into the molecular mechanisms underlying these diseases.By utilizing the Phospho-Smad2 (S465/467) / Smad3 (S423/425) Polyclonal Antibody, researchers can further our understanding of the TGF-beta signaling pathway and its role in disease pathogenesis. This antibody is a crucial tool for studies in cancer biology, immunology, and fibrosis research, ultimately paving the way for the development of novel therapeutic strategies targeting the TGF-beta pathway.
A synthetic phosphorylated peptide around S465 & S467 of human Smad2 (NP_005892.1).
Purification Method:
Affinity purification
Storage Buffer:
Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:
IgG
Sequence:
SSMS
Gene ID:
4087/4088
Uniprot:
Q15796/P84022
Cellular Location:
Cytoplasm, Nucleus
Calculated MW:
48kDa/52kDa/25kDa/35kDa/43kDa
Observed MW:
52kDa/60kDa
Western blot analysis of extracts of HeLa cells, using Phospho-Smad2-S465/467/-Smad3-S423/425 pAb (CABP0548) at 1:1000 dilution or Smad2/Smad3 (CAB7536).HeLa cells were treated by TGF-beta (10ng/mL) for 30 minutes after serum-starvation overnight. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% BSA. Detection: ECL Basic Kit (RM00020). Exposure time: 1s.
Immunohistochemistry of paraffin-embedded Rat brain using Phospho-Smad2-S465/467 + Smad3-S423/425 antibody (CABP0548) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded Human esophagus using Phospho-Smad2-S465/467 + Smad3-S423/425 antibody (CABP0548) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded Mouse kidney using Phospho-Smad2-S465/467 + Smad3-S423/425 antibody (CABP0548) at dilution of 1:100 40x lens).
Immunofluorescence analysis of L929 cells using Phospho-Smad2-S465/467 + Smad3-S423/425 antibody (CABP0548) at dilution of 1:100. Blue: DAPI for nuclear staining.
Synonyms:
HSPC193, HsT17436, JV15-2, LDS1C, LDS3, MADH3
Background:
The protein encoded by this gene belongs to the SMAD, a family of proteins similar to the gene products of the Drosophila gene 'mothers against decapentaplegic' (Mad) and the C. elegans gene Sma. SMAD proteins are signal transducers and transcriptional modulators that mediate multiple signaling pathways. This protein mediates the signal of the transforming growth factor (TGF)-beta, and thus regulates multiple cellular processes, such as cell proliferation, apoptosis, and differentiation. This protein is recruited to the TGF-beta receptors through its interaction with the SMAD anchor for receptor activation (SARA) protein. In response to TGF-beta signal, this protein is phosphorylated by the TGF-beta receptors. The phosphorylation induces the dissociation of this protein with SARA and the association with the family member SMAD4. The association with SMAD4 is important for the translocation of this protein into the nucleus, where it binds to target promoters and forms a transcription repressor complex with other cofactors. This protein can also be phosphorylated by activin type 1 receptor kinase, and mediates the signal from the activin. Alternatively spliced transcript variants have been observed for this gene. [provided by RefSeq, May 2012]
