The Phospho-PTK2 (Y576/577) Polyclonal Antibody (CABP0536) is an essential tool for researchers studying the phosphorylation of PTK2 in cellular signaling pathways. PTK2, also known as focal adhesion kinase (FAK), plays a crucial role in cell adhesion, migration, and survival, making it a key player in cancer progression and metastasis.This antibody, generated in rabbits, specifically targets the phosphorylated form of PTK2 at tyrosine residues 576 and 577, allowing for precise detection and quantification of PTK2 activation. Validated for use in Western blot applications, this antibody is highly reactive with human samples, offering researchers a reliable tool for studying PTK2 phosphorylation dynamics in various cell types.
The phosphorylation of PTK2 at Y576/577 is associated with increased cell motility, invasion, and survival, making it a critical event in cancer development and progression. By studying the regulatory mechanisms and downstream effects of PTK2 phosphorylation, researchers can gain insights into the molecular processes driving cancer metastasis and identify potential therapeutic targets for cancer treatment.Overall, the Phospho-PTK2 (Y576/577) Polyclonal Antibody is a valuable resource for researchers investigating the role of PTK2 phosphorylation in cancer biology, offering new opportunities for understanding and targeting key signaling pathways involved in tumor growth and metastasis.
Product Name:
Phospho-FAK-Y576/577 Rabbit Polyclonal Antibody
SKU:
CABP0536
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human
Immunogen:
A synthetic phosphorylated peptide around Y576 & Y577 of human PTK2 (NP_722560.1).
This gene encodes a cytoplasmic protein tyrosine kinase which is found concentrated in the focal adhesions that form between cells growing in the presence of extracellular matrix constituents. The encoded protein is a member of the FAK subfamily of protein tyrosine kinases but lacks significant sequence similarity to kinases from other subfamilies. Activation of this gene may be an important early step in cell growth and intracellular signal transduction pathways triggered in response to certain neural peptides or to cell interactions with the extracellular matrix. Several transcript variants encoding different isoforms have been found for this gene.
Purification Method:
Affinity purification
Gene ID:
5747
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Western blot analysis of extracts of Jurkat and Raji cells, using Phospho-FAK-Y576/577 antibody (CABP0536) at 1:1000 dilution. Jurkat cells were treated by CD-3 (10 μg/mL) for 2 minutes after serum-starvation overnight or treated by PMA/TPA (200nM) for 10 minutes.Raji cells were treated by PMA/TPA (200nM) for 30 minutes.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% BSA.Detection: ECL Basic Kit (AbGn00020).Exposure time: 90s.
