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Phospho-FAK-Y576/577 Rabbit Polyclonal Antibody (CABP0536)

SKU:
CABP0536
Product Type:
Antibody
Applications:
WB
Reactivity:
Human
Host Species:
Rabbit
Isotype:
IgG
Research Area:
Cardiovascular
€419
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Description

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Product Name:Phospho-FAK-Y576/577 Rabbit Polyclonal Antibody
SKU:CABP0536
Size:20uL, 100uL
Isotype:IgG
Host Species:Rabbit
Reactivity:Human
Immunogen:A synthetic phosphorylated peptide around Y576 & Y577 of human PTK2 (NP_722560.1).
Sequence:STYY KA
Tested Applications:WB ELISA
Recommended Dilution:WB,1:500 - 1:2000
Synonyms:FAK; FADK; FAK1; FRNK; FADK 1; PPP1R71; p125FAK; pp125FAK; Phospho-FAK-Y576/577
Positive Sample:Jurkat,Raji
Conjugate:Unconjugated
Cellular Localization:Cell junction, Cell membrane, Cytoplasm, Cytoplasmic side, Nucleus, Peripheral membrane protein, cell cortex, centrosome, cytoskeleton, focal adhesion, microtubule organizing center.
Calculated MW:119kDa
Observed MW:130kDa

This gene encodes a cytoplasmic protein tyrosine kinase which is found concentrated in the focal adhesions that form between cells growing in the presence of extracellular matrix constituents. The encoded protein is a member of the FAK subfamily of protein tyrosine kinases but lacks significant sequence similarity to kinases from other subfamilies. Activation of this gene may be an important early step in cell growth and intracellular signal transduction pathways triggered in response to certain neural peptides or to cell interactions with the extracellular matrix. Several transcript variants encoding different isoforms have been found for this gene.

Purification Method:Affinity purification
Gene ID:5747
Storage Buffer:Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Product ImageWestern blot analysis of extracts of Jurkat and Raji cells, using Phospho-FAK-Y576/577 antibody (CABP0536) at 1:1000 dilution. Jurkat cells were treated by CD-3 (10 μg/mL) for 2 minutes after serum-starvation overnight or treated by PMA/TPA (200nM) for 10 minutes.Raji cells were treated by PMA/TPA (200nM) for 30 minutes.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% BSA.Detection: ECL Basic Kit (AbGn00020).Exposure time: 90s.

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