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Phospho-PPP2CA/PPP2CB-Y307 Rabbit Polyclonal Antibody (CABP0927)

SKU:
CABP0927
Product Type:
Antibody
Reactivity:
Human
Mouse
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
$503
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Description

system_update_altDatasheetsystem_update_altMSDS
Product Name:Phospho-PPP2CA/PPP2CB-Y307 Rabbit Polyclonal Antibody
SKU:CABP0927
Size:20uL, 100uL
Isotype:IgG
Host Species:Rabbit
Reactivity:Human,Mouse
Immunogen:A synthetic phosphorylated peptide around Y307 of human PPP2CA/PPP2CBPPP2CB (NP_001009552.1).
Sequence:PDYF L
Tested Applications:WB ELISA
Recommended Dilution:WB,1:500 - 1:2000
Synonyms:PPP2CA/PPP2CB; Phospho-PPP2CA/PPP2CB-Y307
Positive Sample:HeLa,NIH/3T3
Cellular Localization:cytosol, extracellular exosome, microtubule cytoskeleton, mitochondrion, nucleus, plasma membrane, spindle pole, synapse
Observed MW:38kDa

This gene encodes the phosphatase 2A catalytic subunit. Protein phosphatase 2A is one of the four major Ser/Thr phosphatases, and it is implicated in the negative control of cell growth and division. It consists of a common heteromeric core enzyme, which is composed of a catalytic subunit and a constant regulatory subunit, that associates with a variety of regulatory subunits. This gene encodes an alpha isoform of the catalytic subunit. [provided by RefSeq, Jul 2008]

Purification Method:Affinity purification
Gene ID:5515/5516
Storage Buffer:Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Product ImageWestern blot analysis of various lysates using Phospho-PPP2CA/PPP2CB-Y307 Rabbit pAb (CABP0927) at 1:1000 dilution.HeLa cells were treated by Calyculin A (100nM) for 30 minutes after serum-starvation overnight.NIH/3T3 cells were treated by Calyculin A (100nM) for 30 minutes after serum-starvation overnight.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% BSA.Detection: ECL Basic Kit (AbGn00020).Exposure time: 1s.

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