The Phospho-PLCG2 (Y759) Polyclonal Antibody (CABP0785) is a reliable tool for researchers studying the phosphorylation of PLCG2 at tyrosine 759. PLCG2 is a key enzyme involved in cellular signaling pathways, particularly in immune cells. This antibody, generated in rabbits, is highly specific to the phosphorylated form of PLCG2 and has been validated for use in various experimental techniques such as Western blotting.Phosphorylation of PLCG2 at tyrosine 759 has been linked to its activation and downstream signaling cascades, making it a crucial event in immune responses and inflammatory processes.
Research involving this phosphorylation site is important for understanding the regulation of PLCG2 activity in diseases such as cancer, autoimmune disorders, and inflammatory conditions. This antibody enables the detection and analysis of phosphorylated PLCG2, providing valuable insights into cellular signaling mechanisms and potential therapeutic targets.
cytoplasm, cytosol, extracellular exosome, perinuclear region of cytoplasm, plasma membrane, ruffle membrane
Calculated MW:
148kDa
Observed MW:
150kDa
The protein encoded by this gene is a transmembrane signaling enzyme that catalyzes the conversion of 1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate to 1D-myo-inositol 1,4,5-trisphosphate (IP3) and diacylglycerol (DAG) using calcium as a cofactor. IP3 and DAG are second messenger molecules important for transmitting signals from growth factor receptors and immune system receptors across the cell membrane. Mutations in this gene have been found in autoinflammation, antibody deficiency, and immune dysregulation syndrome and familial cold autoinflammatory syndrome 3.
Purification Method:
Affinity purification
Gene ID:
5336
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of Raji, using Phospho-PLC gamma 2 (PLCG2)-Y759 Rabbit pAb (CABP0785) at 1:2000 dilution.Raji cells were treated by Pervanadate (1 mM) at 37℃ for 30 minutes.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 1s.
