The Phospho-NF-κB2 (S866) Monoclonal Antibody is a highly specific research tool designed for studying the phosphorylation of NF-κB2 at serine 866. NF-κB2 is a transcription factor that plays a critical role in immune and inflammatory responses by regulating the expression of various genes involved in these processes. This antibody, produced using monoclonal technology, is optimized for use in Western blotting applications and is highly reactive with human samples. It specifically binds to the phosphorylated form of NF-κB2 at serine 866, allowing for the detection and analysis of this key signaling event in various cell types.The phosphorylation of NF-κB2 at serine 866 is known to be involved in the regulation of NF-κB activation and downstream gene expression, making it an important target for research in immunology and cancer biology.
By studying this phosphorylation event, researchers can gain valuable insights into the mechanisms underlying immune and inflammatory responses, as well as potential therapeutic targets for diseases such as cancer and autoimmune disorders. Overall, the Phospho-NF-κB2 (S866) Monoclonal Antibody is a valuable tool for researchers interested in understanding the role of NF-κB2 phosphorylation in immune regulation and inflammatory processes. Its specificity and reliability make it an ideal choice for studies in these areas of research.
Product Name:
Phospho-NF-κB2-S866 Monoclonal Antibody
SKU:
CABP1367
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Mouse
Immunogen:
A synthetic phosphorylated peptide around S866 of human Phospho-NF-κB2-S866 (NP_001070962.1).
This gene encodes a subunit of the transcription factor complex nuclear factor-kappa-B (NFkB). The NFkB complex is expressed in numerous cell types and functions as a central activator of genes involved in inflammation and immune function. The protein encoded by this gene can function as both a transcriptional activator or repressor depending on its dimerization partner. The p100 full-length protein is co-translationally processed into a p52 active form. Chromosomal rearrangements and translocations of this locus have been observed in B cell lymphomas, some of which may result in the formation of fusion proteins. There is a pseudogene for this gene on chromosome 18. Alternative splicing results in multiple transcript variants.
Purification Method:
Affinity purification
Gene ID:
4791
Clone Number:
ARC56344
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.05% proclin300,0.05% BSA,50% glycerol,pH7.3.
Western blot analysis of NIH/3T3, using Phospho-NF-κB2-S866 antibody (CABP1367) at 1:500 dilution.NIH/3T3 cells were treated by TNF-α (20 ng/ml) at 37℃ for 30 minutes.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 30s.
