The Phospho-Histone H1.3 (T17) / Histone H1.4 (T17) Rabbit Monoclonal Antibody (CABP1132) is a powerful tool for researchers studying histone modifications related to gene expression regulation. This antibody specifically recognizes phosphorylated histone H1.3 at threonine 17 and histone H1.4 at threonine 17, crucial modifications that play a role in chromatin structure and genome organization.Histone H1 is a key component of chromatin, acting as a linker histone that stabilizes nucleosome structure. The phosphorylation of histone H1 at specific sites, such as threonine 17 on H1.3 and H1.4, can impact transcriptional regulation and gene expression. By targeting these phosphorylation events, researchers can investigate the dynamic changes in chromatin structure that occur during processes like cell differentiation, development, and disease progression.
This antibody, produced in rabbits, is validated for use in various applications, including Western blotting and immunofluorescence, allowing for the detection and visualization of phospho-histone H1.3 and H1.4 in different cellular contexts. Its high specificity and sensitivity make it an essential tool for studies focused on epigenetic regulation, chromatin dynamics, and gene expression control. By understanding the role of histone H1 phosphorylation in chromatin remodeling, researchers can gain valuable insights into the
Histones are basic nuclear proteins responsible for nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. This gene is intronless and encodes a replication-dependent histone that is a member of the histone H1 family. Transcripts from this gene lack polyA tails but instead contain a palindromic termination element. This gene is found in the large histone gene cluster on chromosome 6.
Purification Method:
Affinity purification
Gene ID:
3007/3008
Clone Number:
ARC0252
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,0.05% BSA,50% glycerol,pH7.3.
Western blot analysis of extracts of various cell lines, using Phospho-Histone H1.3-T17/Histone H1.4-T17 Rabbit mAb (CABP1132) at 1:1000 dilution.Both NIH/3T3 cells and C6 cells were treated by Nocodazole (50 ng/mL) at 37℃ for 20 hours.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% BSA.Detection: ECL Basic Kit (AbGn00020).Exposure time: 1s.
