null

Phospho-eIF4EBP1-T70 Rabbit Polyclonal Antibody (CABP0031)

SKU:
CABP0031
Product Type:
Antibody
Applications:
WB
Reactivity:
Human
Mouse
Rat
Host Species:
Rabbit
Isotype:
IgG
Research Area:
Signal Transduction
€419
Frequently bought together:

Description

system_update_altDatasheetsystem_update_altMSDS
Product Name:Phospho-eIF4EBP1-T70 Rabbit Polyclonal Antibody
SKU:CABP0031
Size:20uL, 100uL
Isotype:IgG
Host Species:Rabbit
Reactivity:Human,Mouse,Rat
Immunogen:A synthetic phosphorylated peptide around T70 of human Phospho-eIF4EBP1-T70 (NP_004086.1).
Sequence:TKTP P
Tested Applications:WB IHC-P ELISA
Recommended Dilution:WB,1:500 - 1:2000 IHC-P,1:50 - 1:200
Synonyms:BP-1; 4EBP1; 4E-BP1; PHAS-I; Phospho-eIF4EBP1-T70
Positive Sample:HeLa
Conjugate:Unconjugated
Cellular Localization:cytoplasm, cytosol
Calculated MW:13kDa
Observed MW:17kDa

This gene encodes one member of a family of translation repressor proteins. The protein directly interacts with eukaryotic translation initiation factor 4E (eIF4E), which is a limiting component of the multisubunit complex that recruits 40S ribosomal subunits to the 5' end of mRNAs. Interaction of this protein with eIF4E inhibits complex assembly and represses translation. This protein is phosphorylated in response to various signals including UV irradiation and insulin signaling, resulting in its dissociation from eIF4E and activation of mRNA translation.

Purification Method:Affinity purification
Gene ID:1978
Storage Buffer:Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Product ImageWestern blot analysis of lysates from HeLa cells, using Phospho-eIF4EBP1-T70 Rabbit pAb (CABP0031) at 1:2000 dilution. HeLa cells were treated by Insulin (100 nM) at 37℃ for 5 minutes after serum-starvation overnight.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% BSA.Detection: ECL Enhanced Kit (AbGn00021).Exposure time: 3min.

Related Products