The Phospho-eIF4EBP1 (T70) Polyclonal Antibody (CABP0031) is a valuable tool for researchers studying the phosphorylation of eIF4EBP1 at T70, a key event in the regulation of protein synthesis. This antibody, generated in rabbits, is highly specific to the phosphorylated form of eIF4EBP1 and is validated for use in Western blot applications.eIF4EBP1 is a crucial player in the control of translation initiation, and its phosphorylation at T70 is known to play a critical role in this process. By targeting the phosphorylated form of eIF4EBP1, researchers can gain insights into the regulation of protein synthesis and its impact on cellular processes such as growth, proliferation, and differentiation.
The Phospho-eIF4EBP1 (T70) Polyclonal Antibody is a valuable tool for studying the role of eIF4EBP1 phosphorylation in various cellular contexts, making it ideal for research in areas such as cancer biology, developmental biology, and molecular signaling pathways. Its high specificity and validated performance make it a reliable choice for scientists looking to explore the intricacies of protein synthesis regulation.
Product Name:
Phospho-eIF4EBP1-T70 Rabbit Polyclonal Antibody
SKU:
CABP0031
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
A synthetic phosphorylated peptide around T70 of human Phospho-eIF4EBP1-T70 (NP_004086.1).
Sequence:
TKTP P
Tested Applications:
WBIHC-PELISA
Recommended Dilution:
WB,1:500 - 1:2000 IHC-P,1:50 - 1:200
Synonyms:
BP-1; 4EBP1; 4E-BP1; PHAS-I; Phospho-eIF4EBP1-T70
Positive Sample:
HeLa
Conjugate:
Unconjugated
Cellular Localization:
cytoplasm, cytosol
Calculated MW:
13kDa
Observed MW:
17kDa
This gene encodes one member of a family of translation repressor proteins. The protein directly interacts with eukaryotic translation initiation factor 4E (eIF4E), which is a limiting component of the multisubunit complex that recruits 40S ribosomal subunits to the 5' end of mRNAs. Interaction of this protein with eIF4E inhibits complex assembly and represses translation. This protein is phosphorylated in response to various signals including UV irradiation and insulin signaling, resulting in its dissociation from eIF4E and activation of mRNA translation.
Purification Method:
Affinity purification
Gene ID:
1978
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Western blot analysis of lysates from HeLa cells, using Phospho-eIF4EBP1-T70 Rabbit pAb (CABP0031) at 1:2000 dilution. HeLa cells were treated by Insulin (100 nM) at 37℃ for 5 minutes after serum-starvation overnight.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% BSA.Detection: ECL Enhanced Kit (AbGn00021).Exposure time: 3min.
