The Phospho-EGFR (Y1045) Polyclonal Antibody (CABP0819) is specifically designed for research related to the phosphorylation of the epidermal growth factor receptor (EGFR) at tyrosine 1045. This antibody, generated in rabbits, exhibits high reactivity with human samples and has been validated for Western blot applications. By binding to the phosphorylated form of EGFR at Y1045, this antibody allows for the detection and analysis of this key signaling molecule in various cell types, making it an excellent tool for studies in cancer biology and signal transduction research.EGFR is a receptor tyrosine kinase that plays a crucial role in regulating cell growth, proliferation, and survival. Phosphorylation of EGFR at specific tyrosine residues, such as Y1045, is known to trigger downstream signaling cascades that influence cellular processes.
The Phospho-EGFR (Y1045) Polyclonal Antibody enables researchers to investigate the phosphorylation status of EGFR at this critical residue, providing valuable insights into the molecular mechanisms underlying EGFR signaling and its implications in cancer development and progression.Understanding the phosphorylation status of EGFR at Y1045 is essential for elucidating the role of this receptor in cancer biology and identifying potential therapeutic targets for cancer treatment. By using the Phospho-EGFR (Y1045) Polyclonal Antibody in their research, scientists can gain valuable information about the
Product Name:
Phospho-EGFR-Y1045 Rabbit Polyclonal Antibody
SKU:
CABP0819
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human
Immunogen:
A synthetic phosphorylated peptide around Y1045 of human EGFR (NP_005219.2).
Cell membrane, Endoplasmic reticulum membrane, Endosome, Endosome membrane, Golgi apparatus membrane, Nucleus membrane, Nucleus, Secreted, Single-pass type I membrane protein.
Calculated MW:
134kDa
Observed MW:
180kDa
The protein encoded by this gene is a transmembrane glycoprotein that is a member of the protein kinase superfamily. This protein is a receptor for members of the epidermal growth factor family. EGFR is a cell surface protein that binds to epidermal growth factor, thus inducing receptor dimerization and tyrosine autophosphorylation leading to cell proliferation. Mutations in this gene are associated with lung cancer. EGFR is a component of the cytokine storm which contributes to a severe form of Coronavirus Disease 2019 (COVID-19) resulting from infection with severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2).
Purification Method:
Affinity purification
Gene ID:
1956
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of lysates from A-431 cells, using Phospho-EGFR-Y1045 Rabbit pAb (CABP0819) at 1:1000 dilution or EGFR antibody (CAB11351). A-431 cells were treated by EGF (100 ng/mL) at 37℃ for 30 minutes after serum-starvation overnight.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% BSA.Detection: ECL Basic Kit (AbGn00020).Exposure time: 1s.
