The Phospho-AMPKa1 (T183) / AMPKa2 (T172) Antibody (CABP1283) is a valuable tool for researchers studying the AMP-activated protein kinase (AMPK) pathway. This antibody, produced in rabbits, is highly specific for detecting phosphorylation of AMPKa1 at threonine 183 and AMPKa2 at threonine 172 in human samples. It has been validated for use in Western blot applications, enabling researchers to study the activation status of AMPK in various cell types.The AMPK pathway plays a crucial role in cellular energy homeostasis and metabolism, making it a key target for research in fields such as metabolic disorders, cancer, and neurodegenerative diseases.
Understanding the phosphorylation status of AMPKa1 and AMPKa2 is essential for unraveling the regulatory mechanisms of AMPK activation and its downstream signaling pathways.By using the Phospho-AMPKa1 (T183) / AMPKa2 (T172) Antibody (CABP1283), researchers can gain insights into the role of AMPK in cellular processes and disease pathogenesis, paving the way for the development of novel therapeutic strategies targeting the AMPK pathway.
The protein encoded by this gene belongs to the ser/thr protein kinase family. It is the catalytic subunit of the 5'-prime-AMP-activated protein kinase (AMPK). AMPK is a cellular energy sensor conserved in all eukaryotic cells. The kinase activity of AMPK is activated by the stimuli that increase the cellular AMP/ATP ratio. AMPK regulates the activities of a number of key metabolic enzymes through phosphorylation. It protects cells from stresses that cause ATP depletion by switching off ATP-consuming biosynthetic pathways. Alternatively spliced transcript variants encoding distinct isoforms have been observed.
Purification Method:
Affinity purification
Gene ID:
5562/5563
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.05% proclin300,50% glycerol,pH7.3.
Western blot analysis of lysates from C6 cells, using Phospho-AMPKa1-T183/AMPKa2-T172 Rabbit pAb (CABP1283) at 1:1000 dilution.C6 cells were treated by AICAR (0.5 mM) at 37℃ for 30 minutes after serum-starvation overnight.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit (AbGn00021).Exposure time: 90s.
