PerCP/Cyanine5.5 Rat IgG2b, κ Isotype Control [LTF-2]
The Percp-Cyanine5.5 Rat IgG2b Isotype Control (LTF-2) is a valuable tool for immunological research, specifically designed for flow cytometry applications. This isotype control antibody, derived from rat IgG2b, serves as a reliable negative control in experiments involving flow cytometry analysis, ensuring accurate interpretation of results by helping to distinguish specific staining from non-specific background signal.Flow cytometry is a powerful technique used in research to analyze and quantify various properties of cells, including surface proteins, cell viability, and cell populations. By using the Percp-Cyanine5.5 Rat IgG2b Isotype Control (LTF-2) alongside experimental samples, researchers can effectively control for non-specific binding of antibodies and confidently identify true positive signals in their flow cytometry assays.
This isotype control antibody is particularly valuable in studies that involve multiple staining protocols or when working with multicolor flow cytometry panels, as it aids in determining the specificity of staining by distinguishing true antigen-positive cells from those showing background staining. Its high quality and specificity make it an essential component for ensuring the accuracy and reliability of flow cytometry data, ultimately contributing to the success of immunological research projects.
Product Name:
PerCP/Cyanine5.5 Rat IgG2b, κ Isotype Control [LTF-2] (AGEL2672)
Product Code:
AGEL2672
Clone No:
LTF-2
Host Species:
Rat
Applications:
FCM
Isotype:
Rat IgG2b, κ
Conjugation:
PerCP/Cyanine5.5
Form:
Liquid
Fluorescent Label:
PerCP/Cyanine5.5 is designed to be excited by the blue laser (488 nm) and detected using an optical filter centered near 675 nm (e.g., a 690/50 nm bandpass filter).
Recommended Use:
Each lot of this antibody is quality control tested by flow cytometric analysis. The amount of the reagent is suggested to be used 5 μL of antibody per test (million cells in 100 µL staining volume or per 100 µL of whole blood). Please check your vial before the experiment. Since applications vary, the appropriate dilutions must be determined for individual use.
Storage Buffer:
Phosphate buffered solution, pH 7.2, containing 0.09% stabilizer and 1% protein protectant.
Stability & Storage:
Keep as concentrated solution. Store at 2-8°C and protected from prolonged exposure to light. Do not freeze. Centrifuge before opening to ensure complete recovery of vial contents. This product is guaranteed up to one year from purchase.