The PE-Cyanine7 Anti-Mouse CD45.2 Antibody (104-2) offered by Assaygenie is a high-quality research tool specially designed for detecting CD45.2, a crucial marker for immune cell subtypes in mice. This antibody is raised against mouse antigens and is validated for use in flow cytometry and immunofluorescence applications.CD45.2 is a member of the protein tyrosine phosphatase family and is essential for T and B cell antigen receptor signaling. It plays a key role in immune cell development, activation, and function. The PE-Cyanine7 Anti-Mouse CD45.
2 Antibody (104-2) enables precise identification and analysis of CD45.2-expressing cells in various mouse tissues and experimental models.With its high specificity and sensitivity, this antibody is an indispensable tool for researchers studying immunology, hematopoiesis, and immune cell phenotyping in mouse models. Its reliable performance and consistent results make it ideal for a wide range of research applications aimed at understanding immune responses, disease mechanisms, and therapeutic interventions in preclinical studies.
Each lot of this antibody is quality control tested by flow cytometric analysis. The amount of the reagent is suggested to be used 5 µL of antibody per test (million cells in 100 µL staining volume or per 100 µL of whole blood). Please check your vial before the experiment. Since applications vary, the appropriate dilutions must be determined for individual use.
Spectrum:
Gene ID:
19264
Storage Buffer:
Phosphate buffered solution, pH 7.2, containing 0.09% stabilizer and 1% protein protectant.
Stability & Storage:
Keep as concentrated solution. Store at 2~8°C and protected from prolonged exposure to light. Do not freeze. Centrifuge before opening to ensure complete recovery of vial contents. This product is guaranteed up to one year from purchase.
Background:
CD45.2 is an alloantigen of CD45, expressed by Ly5.2 bearing mouse strains (e.g., A, AKR, BALB/c, CBA/Ca, CBA/J, C3H/He, C57BL, C57BR, C57L, C58, DBA/1, DBA/2, NZB, SWR, 129). CD45, a member of the protein tyrosine phosphatase (PTP) family, is a 180-240 kD glycoprotein expressed on all hematopoietic cells except mature erythrocytes and platelets. There are multiple isoforms in the mouse that play key roles in TCR and BCR signal transduction. These isoforms are very specific to the activation and maturation states of the cell as well as specific cell type. The primary ligands for CD45 are galectin-1, CD2, CD3, CD4, TCR, CD22, and Thy-1.