The NFU1 Polyclonal Antibody (CAB7097) is a vital tool for researchers studying NFU1, a protein involved in iron-sulfur cluster biogenesis and maintenance. This antibody, produced in rabbits, exhibits high reactivity with human samples and has been validated for use in Western blot applications. By specifically binding to NFU1, researchers can accurately detect and analyze this protein in a variety of cell types, making it ideal for investigations in biochemistry, mitochondrial biology, and genetic disorders related to iron metabolism.
NFU1 is a crucial component of the mitochondrial machinery responsible for producing and delivering iron-sulfur clusters to various proteins involved in key cellular processes like energy production and DNA repair. Dysregulation of NFU1 function is associated with severe genetic disorders, highlighting its importance in cellular health and metabolism. Research into NFU1 is essential for unraveling the mechanisms underlying these diseases and developing targeted therapies to treat them.
Product Name:
NFU1 Rabbit Polyclonal Antibody
SKU:
CAB7097
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-230 of human NFU1 (NP_001002755.1).
This gene encodes a protein that is localized to mitochondria and plays a critical role in iron-sulfur cluster biogenesis. The encoded protein assembles and transfers 4Fe-4S clusters to target apoproteins including succinate dehydrogenase and lipoic acid synthase. Mutations in this gene are a cause of multiple mitochondrial dysfunctions syndrome-1, and pseudogenes of this gene are located on the short arms of chromosomes 1 and 3. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene.
Purification Method:
Affinity purification
Gene ID:
27247
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Western blot analysis of extracts of various cell lines, using NFU1 antibody (CAB7097) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 30s.
