The MRPL35 Polyclonal Antibody (PACO07202) is a valuable tool for research involving MRPL35, a protein involved in mitochondrial ribosomal assembly and protein synthesis. This antibody, produced using rabbits, demonstrates high reactivity specifically with human samples and has been validated for use in Western blot applications. By binding to the MRPL35 protein, this antibody enables accurate detection and analysis in various cell types, making it ideal for studies in mitochondrial biology, metabolism, and cancer research.MRPL35 is a key component of the mitochondrial ribosome, playing a crucial role in the synthesis of proteins essential for mitochondrial function.
Dysregulation of MRPL35 has been implicated in various diseases, including metabolic disorders and cancer. Therefore, understanding the function and regulation of MRPL35 is vital for uncovering potential therapeutic targets for treating these conditions.Overall, the MRPL35 Polyclonal Antibody is a reliable tool for researchers interested in investigating the role of MRPL35 in mitochondrial protein synthesis and its implications in human health and disease.
Antibody Name:
MRPL35 Antibody (PACO07202)
Antibody SKU:
PACO07202
Size:
50ug
Host Species:
Rabbit
Tested Applications:
ELISA, WB
Recommended Dilutions:
ELISA:1:10000, WB:1:500-1:2000
Species Reactivity:
Human, Mouse
Immunogen:
Synthesized peptide derived from the Internal region of human MRP-L35. at AA rangle: 80-160
Form:
Liquid
Storage Buffer:
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Purification Method:
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Clonality:
Polyclonal
Isotype:
IgG
Conjugate:
Non-conjugated
Western blot analysis of 293 using MRP-L35 antibody. Antibody was diluted at 1:2000. Secondary antibody was diluted at 1:20000.
Western blot analysis of 293T COLO using MRP-L35 antibody. Antibody was diluted at 1:2000. Secondary antibody was diluted at 1:20000.
Synonyms:
MRPL35; BM-007; 39S ribosomal protein L35, mitochondrial; L35mt; MRP-L35
UniProt Protein Function:
MRPL35: a mitochondrial ribosomal protein encoded by a nuclear gene that helps in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. This gene encodes a 39S subunit protein. Sequence analysis identified three transcript variants. Pseudogenes corresponding to this gene are found on chromosomes 6p, 10q, and Xp. [provided by RefSeq, Jul 2008]Protein type: Translation; MitochondrialChromosomal Location of Human Ortholog: 2p11.2Cellular Component: mitochondrial inner membrane; mitochondrial ribosome; mitochondrionMolecular Function: structural constituent of ribosomeBiological Process: mitochondrial translation; organelle organization and biogenesis; translation
UniProt Protein Details:
NCBI Summary:
Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. This gene encodes a 39S subunit protein. Sequence analysis identified three transcript variants. Pseudogenes corresponding to this gene are found on chromosomes 6p, 10q, and Xp. [provided by RefSeq, Jul 2008]
