The MRPL28 Polyclonal Antibody (CAB5897) is a valuable tool for researchers studying mitochondrial ribosomal protein L28 (MRPL28), a protein involved in mitochondrial protein synthesis. This antibody, produced in rabbits, exhibits high reactivity with human samples and is validated for use in Western blot applications. By binding specifically to the MRPL28 protein, this antibody enables the detection and analysis of MRPL28 in various cell types, making it suitable for research in the fields of mitochondrial biology and cancer research.
MRPL28 is a key component of the mitochondrial ribosome, where it plays a crucial role in protein translation within the mitochondria. Dysregulation of MRPL28 has been implicated in various diseases, including cancer and mitochondrial disorders, highlighting the importance of studying its function. By using the MRPL28 Polyclonal Antibody in research, scientists can gain a better understanding of the role of MRPL28 in mitochondrial protein synthesis and its potential implications in disease pathology.
Product Name:
MRPL28 Rabbit Polyclonal Antibody
SKU:
CAB5897
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-256 of human MRPL28 (NP_006419.2).
WB,1:500 - 1:2000 IHC-P,1:50 - 1:200 IF/ICC,1:50 - 1:100 IP,0.5μg-4μg antibody for 200μg-400μg extracts of whole cells
Synonyms:
p15; MAAT1; MRPL28
Positive Sample:
MCF7,HepG2,293T,A-549
Conjugate:
Unconjugated
Cellular Localization:
Mitochondrion.
Calculated MW:
30kDa
Observed MW:
30kDa
Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. This gene encodes a 39S subunit protein, a part of which was originally isolated by its ability to recognize tyrosinase in an HLA-A24-restricted fashion.
Purification Method:
Affinity purification
Gene ID:
10573
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Western blot analysis of various lysates using MRPL28 Rabbit pAb (CAB5897) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).
