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Mouse Retinoid X Receptor Alpha (RXRa) ELISA Kit

SKU:
MODL01138
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
P28700
Sensitivity:
0.067ng/mL
Range:
0.156-10ng/mL
ELISA Type:
Sandwich
Synonyms:
NR2B1,RXR-A,NR2-B1,Nuclear Receptor Subfamily 2,Group B,Member 1,Retinoic acid receptor RXR-alpha
Reactivity:
Mouse
Sample Type:
Tissue Homogenates And Other Biological Fluids
€649
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Description

system_update_altDatasheet

Mouse Retinoid X Receptor Alpha (RXRa) ELISA Kit

The Mouse Retinoid X Receptor Alpha (RXRA) ELISA Kit is a reliable and accurate assay designed for the quantitative measurement of RXRA levels in mouse serum, plasma, and cell lysates. This kit offers high sensitivity and specificity, with a wide dynamic range, allowing for precise and reproducible results in various research applications.RXRA is a key nuclear receptor involved in regulating gene expression and plays a crucial role in diverse biological processes such as metabolism, immunity, and development.

Dysregulation of RXRA has been implicated in various diseases including diabetes, inflammation, and cancer, making it a valuable target for studying disease mechanisms and potential therapeutic interventions.Overall, the Mouse Retinoid X Receptor Alpha (RXRA) ELISA Kit provides researchers with a powerful tool to study the role of RXRA in health and disease, offering insights into potential diagnostic and therapeutic strategies.

Product Name:Mouse Retinoid X Receptor Alpha (RXRa) ELISA Kit
Product Code:MODL01138
Size:96 Assays
Target:RXRa
Synonyms:NR2B1, RXR-A, NR2-B1, Nuclear Receptor Subfamily 2,Group B,Member 1, Retinoic acid receptor RXR-alpha
Detection Method:Sandwich
Range:0.156-10ng/mL
Shelf Life:12 months
Note:For research use only
Recovery:Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
MatrixRecovery range (%)Average(%)
Serum (n=5)80-10291
EDTA plasma (n=5)81-10090
Heparin plasma (n=5)80-8984
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
Serum (n=5) 82-96% 83-98% 81-99% 93-101%
EDTA plasma (n=5) 88-101% 86-95% 90-102% 80-93%
Heparin plasma (n=5) 80-91% 82-90% 95-104% 79-95%
Intra-assay Precision:Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
StepProtocol
1. Prepare all reagents, samples and standards
2.Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.Aspirate and wash 3 times
5.Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.Aspirate and wash 5 times
7.Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.Add 50µL Stop Solution. Read at 450nm immediately.

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