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Mouse Regulator Of G Protein Signaling 6 (RGS6) ELISA Kit

SKU:
MODL01125
Product Type:
ELISA Kit
Sensitivity:
0.066ng/mL
Range:
0.156-10ng/mL
ELISA Type:
Sandwich
Synonyms:
GAP,S914
Reactivity:
Mouse
Sample Type:
Tissue Homogenates And Other Biological Fluids
$779
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Description

system_update_altDatasheet

Mouse Regulator Of G Protein Signaling 6 (RGS6) ELISA Kit

The Mouse Regulator of G-Protein Signaling 6 (RGS6) ELISA Kit is a powerful tool for the precise measurement of RGS6 levels in mouse serum, plasma, and tissue samples. This kit offers exceptional sensitivity and specificity, guaranteeing accurate and reproducible results for a variety of research purposes.RGS6 is a key regulator of G-protein signaling pathways, playing a critical role in modulating cell signaling and physiological processes. Dysregulation of RGS6 has been implicated in various diseases, including neurological disorders, cardiovascular conditions, and cancer.

By accurately measuring RGS6 levels, researchers can gain valuable insights into the mechanisms underlying these diseases and potentially identify new therapeutic targets. The Mouse RGS6 ELISA Kit is an essential tool for studying the role of RGS6 in health and disease, advancing our understanding of signaling pathways and potential treatment strategies.

Product Name:Mouse Regulator Of G Protein Signaling 6 (RGS6) ELISA Kit
Product Code:MODL01125
Size:96 Assays
Target:RGS6
Synonyms:GAP, S914
Detection Method:Sandwich
Range:0.156-10ng/mL
Shelf Life:12 months
Note:For research use only
Recovery:Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
MatrixRecovery range (%)Average(%)
Serum (n=5)80-10291
EDTA plasma (n=5)81-10090
Heparin plasma (n=5)80-8984
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
Serum (n=5) 82-96% 83-98% 81-99% 93-101%
EDTA plasma (n=5) 88-101% 86-95% 90-102% 80-93%
Heparin plasma (n=5) 80-91% 82-90% 95-104% 79-95%
Intra-assay Precision:Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
StepProtocol
1. Prepare all reagents, samples and standards
2.Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.Aspirate and wash 3 times
5.Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.Aspirate and wash 5 times
7.Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.Add 50µL Stop Solution. Read at 450nm immediately.

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