Mouse Regulated On Activation In Normal T-Cell Expressed And Secreted (RANTES) ELISA Kit
- SKU:
- MODL01113
- Product Type:
- ELISA Kit
- Size:
- 96 Assays
- Uniprot:
- P30882
- Sensitivity:
- 31pg/mL
- Range:
- 78.125-5000pg/mL
- ELISA Type:
- Sandwich
- Synonyms:
- CCL5,SCYA5,SISd,EoCP,Chemokine C-C-Motif Ligand 5,Eosinophil Chemotactic Cytokine,T-cell Specific Protein p288,Small Inducible Cytokine Subfamily A(Cys-Cys)Member 5
- Reactivity:
- Mouse
- Sample Type:
- Serum, Plasma, Tissue Homogenates, Cell Lysates, Cell Culture Supernatants And Other Biological Fluids
Frequently bought together:
Description
system_update_altDatasheet
Mouse Regulated On Activation In Normal T-Cell Expressed And Secreted (RANTES) ELISA Kit
The Mouse Regulated on Activation, Normal T Cell Expressed and Secreted (RANTES) ELISA Kit is a powerful tool for detecting RANTES levels in mouse serum, plasma, and cell culture supernatants. With its high sensitivity and specificity, this kit provides accurate and reliable results for a variety of research applications.RANTES is a key chemokine involved in inflammatory responses and immune regulation. It plays a crucial role in recruiting immune cells to sites of infection or injury, as well as in the development of inflammatory diseases.
By measuring RANTES levels, researchers can gain valuable insights into the immune response and potential therapeutic targets for various diseases.This ELISA kit is easy to use, with clear instructions and quick results, making it an essential tool for studying immune responses, inflammation, and infectious diseases in mouse models. Trust in the Mouse RANTES ELISA Kit for precise and dependable measurements of RANTES levels in your research.
| Product Name: | Mouse Regulated On Activation In Normal T-Cell Expressed And Secreted (RANTES) ELISA Kit |
| Product Code: | MODL01113 |
| Size: | 96 Assays |
| Target: | RANTES |
| Synonyms: | CCL5, SCYA5, SISd, EoCP, Chemokine C-C-Motif Ligand 5, Eosinophil Chemotactic Cytokine, T-cell Specific Protein p288, Small Inducible Cytokine Subfamily A(Cys-Cys)Member 5 |
| Detection Method: | Sandwich |
| Range: | 78.1-5,000pg/mL |
| Shelf Life: | 12 months |
| Note: | For research use only |
| Recovery: | Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples. | ||||||||||||||||||||
| |||||||||||||||||||||
| Linearity: | The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected. | ||||||||||||||||||||
| |||||||||||||||||||||
| Intra-assay Precision: | Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively. | ||||||||||||||||||||
| Inter-assay Precision: | Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate. | ||||||||||||||||||||
| Stability: | The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end. |
| Step | Protocol |
| 1. | Prepare all reagents, samples and standards |
| 2. | Add 100µL standard or sample to each well. Incubate 2 hours at 37°C |
| 3. | Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C |
| 4. | Aspirate and wash 3 times |
| 5. | Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C |
| 6. | Aspirate and wash 5 times |
| 7. | Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C |
| 8. | Add 50µL Stop Solution. Read at 450nm immediately. |
Related Products
Canine Regulated On Activation In Normal T-Cell Expressed And Secreted (RANTES) ELISA Kit
$779
Bovine Regulated On Activation In Normal T-Cell Expressed And Secreted (RANTES) ELISA Kit
$899
Porcine Regulated On Activation In Normal T-Cell Expressed And Secreted (RANTES) ELISA Kit
$899
Mouse RANTES (Regulated On Activation, Normal T-Cell Expressed and Secreted) ELISA Kit - High Sensitivity
$599
Rat Regulated On Activation In Normal T-Cell Expressed And Secreted (RANTES) ELISA Kit
$779
Human Regulated On Activation In Normal T-Cell Expressed And Secreted (RANTES) ELISA Kit
$719
Guinea pig Regulated On Activation In Normal T-Cell Expressed And Secreted (RANTES) ELISA Kit
$779