Mouse PIN1 Recombinant Protein (RPPB2114)
- SKU:
- RPPB2114
- Product Type:
- Recombinant Protein
- Species:
- Mouse
- Uniprot:
- Q9QUR7
- Research Area:
- Enzymes
Description
| Product Name: | Mouse PIN1 Recombinant Protein |
| Product Code: | RPPB2114 |
| Size: | 10µg |
| Species: | Mouse |
| Target: | PIN1 |
| Synonyms: | Peptidyl-prolyl cis-trans isomerase NIMA-interacting 1 (EC:5.2.1.8), Peptidyl-prolyl cis-trans isomerase Pin1, PPIase Pin1, Pin1, PIN1. |
| Source: | Escherichia Coli |
| Physical Appearance: | Sterile Filtered clear solution. |
| Formulation: | PIN1 protein solution (1mg/ml) containing Phosphate buffered saline (pH7.4) and 10% glycerol. |
| Stability: | Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles. |
| Purity: | Greater than 95.0% as determined by SDS-PAGE. |
| Amino Acid Sequence: | MGSSHHHHHH SSGLVPRGSH MGSMADEEKL PPGWEKRMSR SSGRVYYFNH ITNASQWERP SGGSTVGGSS KNGQGEPAKV RCSHLLVKHS QSRRPSSWRQ EKITRSKEEA LELINGYIQK IKSGEEDFES LASQFSDCSS AKARGDLGPF SRGQMQKPFE DASFALRTGE MSGPVFTDSG IHIILRTE |
| Biological Activity: | Specific activity is > 1,200 nmol/min/mg, and is defined as the amount of enzyme that cleaves 1nmole of suc-AAFP-PNA per minute at 37�C in Tris-HCl pH 8.0 using chymotrypsin. |
Pin 1 is a peptidyl-prolyl cis/trans isomerase (PPIase) which�interacts with NIMA and essential for cell cycle regulation Pin1 is nuclear PPIase containing a WW protein interaction domain, and is structurally and functionally related to Ess1/Ptf1, an essential protein in budding yeast. PPIase activity is necessary for Ess1/Pin1 function in yeast. Pin1 is thus an essential PPIase that regulates mitosis presumably by interacting with NIMA and attenuating its mitosis-promoting activity. Substrates of Pin1 include the mitotic regulators (Cdc25 phosphatase and NIMA, PLK I, Wee, and Myt1 kinases), several transcription factors like b-Catenin, c-Jun, and the tumor suppressor protein p53, and some specific proteins like the RNA Pol II, the cytoskeleton protein tau, and the G1/S protein Cyclin D1.
PIN1 Mouse Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 188 amino acids (1-165 a.a) and having a molecular mass of 20.8kDa. PIN1 is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
| UniProt Protein Function: | Peptidyl-prolyl cis/trans isomerase (PPIase) that binds to and isomerizes specific phosphorylated Ser/Thr-Pro (pSer/Thr-Pro) motifs. By inducing conformational changes in a subset of phosphorylated proteins, acts as a molecular switch in multiple cellular processes. Displays a preference for an acidic residue N-terminal to the isomerized proline bond. Regulates mitosis presumably by interacting with NIMA and attenuating its mitosis-promoting activity. Down-regulates kinase activity of BTK. Can transactivate multiple oncogenes and induce centrosome amplification, chromosome instability and cell transformation. Required for the efficient dephosphorylation and recycling of RAF1 after mitogen activation (). Binds and targets PML and BCL6 for degradation in a phosphorylation-dependent manner (PubMed:17828269). Acts as a regulator of JNK cascade by binding to phosphorylated FBXW7, disrupting FBXW7 dimerization and promoting FBXW7 autoubiquitination and degradation: degradation of FBXW7 leads to subsequent stabilization of JUN (). May facilitate the ubiquitination and proteasomal degradation of RBBP8/CtIP through CUL3/KLHL15 E3 ubiquitin-protein ligase complex, hence favors DNA double-strand repair through error-prone non-homologous end joining (NHEJ) over error-free, RBBP8-mediated homologous recombination (HR) (). |
| UniProt Protein Details: | |
| NCBI Summary: | |
| UniProt Code: | Q9QUR7 |
| NCBI GenInfo Identifier: | 20139259 |
| NCBI Gene ID: | 23988 |
| NCBI Accession: | Q9QUR7.1 |
| UniProt Secondary Accession: | Q9QUR7 |
| UniProt Related Accession: | Q9QUR7 |
| Molecular Weight: | |
| NCBI Full Name: | Peptidyl-prolyl cis-trans isomerase NIMA-interacting 1 |
| NCBI Synonym Full Names: | protein (peptidyl-prolyl cis/trans isomerase) NIMA-interacting 1 |
| NCBI Official Symbol: | Pin1�� |
| NCBI Official Synonym Symbols: | D9Bwg1161e; 0610025L01Rik�� |
| NCBI Protein Information: | peptidyl-prolyl cis-trans isomerase NIMA-interacting 1 |
| UniProt Protein Name: | Peptidyl-prolyl cis-trans isomerase NIMA-interacting 1 |
| UniProt Synonym Protein Names: | Peptidyl-prolyl cis-trans isomerase Pin1; PPIase Pin1 |
| Protein Family: | Peptidyl-prolyl cis-trans isomerase |
| UniProt Gene Name: | Pin1�� |
| UniProt Entry Name: |
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