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Mouse Matrix Metalloproteinase 19 (MMP19) ELISA Kit

SKU:
MODL00877
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
Q9JHI0
Sensitivity:
0.054ng/mL
Range:
0.156-10ng/mL
ELISA Type:
Sandwich
Synonyms:
RASI-1,MMP18,Occasionally Referred To As Stromelysin-4
Reactivity:
Mouse
Sample Type:
Tissue Homogenates, Cell Lysates And Other Biological Fluids
€649
Frequently bought together:

Description

system_update_altDatasheet

Mouse Matrix Metalloproteinase 19 (MMP19) ELISA Kit

The Mouse Matrix Metalloproteinase-19 (MMP19) ELISA Kit is specifically designed for the accurate detection of MMP19 levels in mouse serum, plasma, and cell culture supernatants. This kit offers high sensitivity and specificity, ensuring precise and reliable results for a variety of research applications.MMP19 is a key enzyme in the degradation of extracellular matrix proteins, playing a crucial role in tissue remodeling and wound healing. Dysregulation of MMP19 has been implicated in various pathological conditions such as cancer, inflammation, and fibrosis, making it a valuable biomarker for studying these diseases and potential therapeutic interventions.

With its advanced technology and superior performance, the Mouse MMP19 ELISA Kit from Assaygenie is an essential tool for researchers investigating the role of MMP19 in physiological and pathological processes in mouse models.

Product Name:Mouse Matrix Metalloproteinase 19 (MMP19) ELISA Kit
Product Code:MODL00877
Size:96 Assays
Target:MMP19
Synonyms:RASI-1, MMP18, Occasionally Referred To As Stromelysin-4
Detection Method:Sandwich
Range:0.156-10ng/mL
Shelf Life:12 months
Note:For research use only
Recovery:Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
MatrixRecovery range (%)Average(%)
Serum (n=5)83-10091
EDTA plasma (n=5)90-10396
Heparin plasma (n=5)89-10295
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
Serum (n=5) 82-96% 83-98% 81-99% 93-101%
EDTA plasma (n=5) 88-101% 86-95% 90-102% 80-93%
Heparin plasma (n=5) 80-91% 82-90% 95-104% 79-95%
Intra-assay Precision:Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
StepProtocol
1. Prepare all reagents, samples and standards
2.Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.Aspirate and wash 3 times
5.Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.Aspirate and wash 5 times
7.Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.Add 50µL Stop Solution. Read at 450nm immediately.

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