The MAPKAPK3 Polyclonal Antibody is a valuable tool for research involving MAPKAPK3, a protein kinase involved in cellular responses to stress and inflammation. This antibody, raised in rabbits, is highly specific and reactive with human samples, making it ideal for use in Western blot applications. By targeting the MAPKAPK3 protein, researchers can effectively study its role in various cellular processes, particularly in the context of immune regulation and inflammatory mechanisms.MAPKAPK3, also known as MK3, is a crucial player in signaling pathways that regulate cell growth, differentiation, and survival.
Its activation is linked to various diseases, including cancer, inflammatory disorders, and neurodegenerative conditions. By utilizing the MAPKAPK3 Polyclonal Antibody, researchers can gain insights into the function of this kinase and its potential as a therapeutic target for such diseases. Understanding the molecular mechanisms involving MAPKAPK3 is essential for developing targeted therapies that may improve patient outcomes in these contexts.
Product Name:
MAPKAPK3 Polyclonal Antibody
SKU:
CAB21655
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-240 of human MAPKAPK3 (NP_004626.1).
This gene encodes a member of the Ser/Thr protein kinase family. This kinase functions as a mitogen-activated protein kinase (MAP kinase)- activated protein kinase. MAP kinases are also known as extracellular signal-regulated kinases (ERKs), act as an integration point for multiple biochemical signals. This kinase was shown to be activated by growth inducers and stress stimulation of cells. In vitro studies demonstrated that ERK, p38 MAP kinase and Jun N-terminal kinase were all able to phosphorylate and activate this kinase, which suggested the role of this kinase as an integrative element of signaling in both mitogen and stress responses. This kinase was reported to interact with, phosphorylate and repress the activity of E47, which is a basic helix-loop-helix transcription factor known to be involved in the regulation of tissue-specific gene expression and cell differentiation. Alternate splicing results in multiple transcript variants that encode the same protein.
Purification Method:
Affinity purification
Gene ID:
7867
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Western blot analysis of extracts of various cell lines, using MAPKAPK3 antibody (CAB21655) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit (AbGn00021).Exposure time: 90s.
