LATS1 Monoclonal Antibody (CAB22287)
- SKU:
- CAB22287
- Product Type:
- Antibody
- Antibody Type:
- Monoclonal Antibody
- Reactivity:
- Human
- Host Species:
- Rabbit
- Isotype:
- IgG
- Applications:
- WB
Description
| Product Name: | LATS1 Monoclonal Antibody |
| SKU: | CAB22287 |
| Size: | 20uL, 100uL |
| Isotype: | IgG |
| Host Species: | Rabbit |
| Reactivity: | Human |
| Immunogen: | A synthetic peptide corresponding to a sequence within amino acids 1-100 of human LATS1 (NP_004681.1). |
| Sequence: | MKRS EKPE GYRQ MRPK TFPA SNYT VSSR QMLQ EIRE SLRN LSKP SDAA KAEH NMSK MSTE DPRQ VRNP PKFG THHK ALQE IRNS LLPF ANET NSSR STSE |
| Tested Applications: | WB ELISA |
| Recommended Dilution: | WB,1:1000 - 1:5000 |
| Synonyms: | wts; WARTS; LATS1 |
| Positive Sample: | SH-SY5Y |
| Conjugate: | Unconjugated |
| Cellular Localization: | Cytoplasm, centrosome, cytoskeleton, microtubule organizing center. |
| Calculated MW: | 127kDa |
| Observed MW: | 150kDa |
The protein encoded by this gene is a putative serine/threonine kinase that localizes to the mitotic apparatus and complexes with cell cycle controller CDC2 kinase in early mitosis. The protein is phosphorylated in a cell-cycle dependent manner, with late prophase phosphorylation remaining through metaphase. The N-terminal region of the protein binds CDC2 to form a complex showing reduced H1 histone kinase activity, indicating a role as a negative regulator of CDC2/cyclin A. In addition, the C-terminal kinase domain binds to its own N-terminal region, suggesting potential negative regulation through interference with complex formation via intramolecular binding. Biochemical and genetic data suggest a role as a tumor suppressor. This is supported by studies in knockout mice showing development of soft-tissue sarcomas, ovarian stromal cell tumors and a high sensitivity to carcinogenic treatments.
| Purification Method: | Affinity purification |
| Gene ID: | 9113 |
| Clone Number: | ARC56891 |
| Storage Buffer: | Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.05% proclin300,0.05% BSA,50% glycerol,pH7.3. |
 | Western blot analysis of SH-SY5Y, using LATS1 antibody (CAB22287) at1:2000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 90s. |
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