The KO Validated YAP1 Polyclonal Antibody (CAB1002) is a powerful tool for studying the YAP1 protein, a key regulator of cell growth and proliferation. This antibody, produced in rabbits, has been rigorously tested and validated for use in a variety of applications, including Western blot analysis.YAP1 plays a critical role in the Hippo signaling pathway, controlling cell differentiation, organ size, and tumorigenesis. Dysregulation of YAP1 has been implicated in numerous diseases, including cancer and developmental disorders. By targeting the YAP1 protein, researchers can gain insight into the molecular mechanisms underlying these conditions and develop potential therapeutic interventions.
With its high specificity and sensitivity, the KO Validated YAP1 Polyclonal Antibody is an essential tool for investigating the function of YAP1 in various cellular processes. Whether studying normal development or disease pathology, this antibody enables accurate detection and analysis of the YAP1 protein, paving the way for new discoveries in cell biology and oncology research.
Product Name:
YAP1 Polyclonal Antibody
SKU:
CAB1002
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 155-504 of human YAP1 (NP_001123617.1).
WB,1:2000 - 1:20000 IHC-P,1:500 - 1:1000 IF/ICC,1:50 - 1:200 IP,0.5μg-4μg antibody for 200μg-400μg extracts of whole cells
Synonyms:
YAP; YKI; COB1; YAP2; YAP-1; YAP65; YAP1
Positive Sample:
HeLa
Conjugate:
Unconjugated
Cellular Localization:
Cytoplasm, Nucleus.
Calculated MW:
54kDa
Observed MW:
70kDa
This gene encodes a downstream nuclear effector of the Hippo signaling pathway which is involved in development, growth, repair, and homeostasis. This gene is known to play a role in the development and progression of multiple cancers as a transcriptional regulator of this signaling pathway and may function as a potential target for cancer treatment. Alternative splicing results in multiple transcript variants encoding different isoforms.
Purification Method:
Affinity purification
Gene ID:
10413
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of lysates from wild type (WT) and YAP1 knockout (KO) HeLa cells using YAP1 Rabbit pAb (CAB1002) at 1:2000 dilution.Secondary antibody:HRP Goat Anti-Rabbit IgG (H+L)(CABS014) at 1:10000 dilution.Lysates/proteins: 25 μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection:ECL Basic Kit (AbGn00020).Exposuretime: 30s.
