The KO Validated CDK2 Rabbit Polyclonal Antibody (CAB18000) is a powerful tool for research involving CDK2, a critical protein involved in cell cycle regulation and cell proliferation. This antibody, raised in rabbits, is highly specific for CDK2 and has been validated for knockout (KO) studies, ensuring accurate and reliable results.CDK2, a member of the cyclin-dependent kinase family, plays a crucial role in regulating the cell cycle by controlling the progression from G1 to S phase. Dysregulation of CDK2 has been implicated in various diseases, including cancer, making it an attractive target for therapeutic intervention.
This antibody is ideal for use in Western blot applications, allowing for the detection and analysis of CDK2 protein levels in various cell types and experimental conditions. Its high specificity and sensitivity make it a valuable tool for researchers studying cell cycle regulation, cancer biology, and therapeutic targeting of CDK2 for treatment development.
Product Name:
[KO Validated] CDK2 Rabbit Polyclonal Antibody
SKU:
CAB18000
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
A synthetic peptide corresponding to a sequence within amino acids 200-298 of human CDK2 (NP_001789.2).
This gene encodes a member of a family of serine/threonine protein kinases that participate in cell cycle regulation. The encoded protein is the catalytic subunit of the cyclin-dependent protein kinase complex, which regulates progression through the cell cycle. Activity of this protein is especially critical during the G1 to S phase transition. This protein associates with and regulated by other subunits of the complex including cyclin A or E, CDK inhibitor p21Cip1 (CDKN1A), and p27Kip1 (CDKN1B). Alternative splicing results in multiple transcript variants.
Purification Method:
Affinity purification
Gene ID:
1017
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of lysates from wild type (WT) and CDK2 knockout (KO) 293T cells, using [KO Validated] CDK2 Rabbit pAb (CAB18000) at 1:3000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 5s.
