The INTS4 Polyclonal Antibody (CAB6339) is a valuable tool for researchers studying the INTS4 protein, a key component of the integrator complex involved in controlling gene expression. This antibody, raised in rabbits, demonstrates high reactivity with human samples and is optimized for use in Western blot applications. By specifically binding to the INTS4 protein, this antibody enables precise detection and analysis in a variety of cell types, making it a versatile tool for studies in molecular biology and gene regulation.INTS4 plays a crucial role in transcriptional regulation by facilitating the processing of small nuclear RNAs and promoting mRNA formation.
Its involvement in gene expression control makes INTS4 a target of interest in research on various biological processes, including development, cell differentiation, and disease progression. Investigating the function of INTS4 with this antibody can provide valuable insights into the mechanisms underlying gene regulation and open new avenues for therapeutic interventions in conditions related to aberrant gene expression.
Product Name:
INTS4 Rabbit Polyclonal Antibody
SKU:
CAB6339
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-270 of human INTS4 (NP_291025.3).
WB,1:500 - 1:1000 IP,0.5μg-4μg antibody for 200μg-400μg extracts of whole cells
Synonyms:
INT4; MST093; INTS4
Positive Sample:
HepG2,Jurkat,Mouse testis,Rat liver
Conjugate:
Unconjugated
Cellular Localization:
Nucleus.
Calculated MW:
108kDa
Observed MW:
108kDa
INTS4 is a subunit of the Integrator complex, which associates with the C-terminal domain of RNA polymerase II large subunit (POLR2A; MIM 180660) and mediates 3-prime end processing of small nuclear RNAs U1 (RNU1; MIM 180680) and U2 (RNU2; MIM 180690) (Baillat et al., 2005 [PubMed 16239144]).
Purification Method:
Affinity purification
Gene ID:
92105
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Western blot analysis of various lysates using INTS4 Rabbit pAb (CAB6339) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 1s.
