Human SRSF1 Recombinant Protein (RPPB4821)
- SKU:
- RPPB4821
- Product Type:
- Recombinant Protein
- Species:
- Human
- Uniprot:
- Q07955
Description
| Product Name: | Human SRSF1 Recombinant Protein |
| Product Code: | RPPB4821 |
| Size: | 20µg |
| Species: | Human |
| Target: | SRSF1 |
| Synonyms: | ASF, MGC5228, SF2, SF2p33, SRp30a, Serine/arginine-rich splicing factor 1, SRSF1, splicing factor 1, ASF-1, Splicing factor, arginine/serine-rich 1, pre-mRNA-splicing factor SF2, P33 subunit, OK/SW-cl.3. |
| Source: | Escherichia Coli |
| Physical Appearance: | Sterile Filtered colorless solution. |
| Formulation: | The SRSF1 solution (1mg/ml) contains 20mM Tris-HCl buffer (pH 8.0), 0.4M urea and 10% glycerol. |
| Stability: | Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles. |
| Purity: | Greater than 90% as determined by SDS-PAGE. |
| Amino Acid Sequence: | MGSSHHHHHH SSGLVPRGSH MSGGGVIRGP AGNNDCRIYV GNLPPDIRTK DIEDVFYKYG AIRDIDLKNR RGGPPFAFVE FEDPRDAEDA VYGRDGYDYD GYRLRVEFPR SGRGTGRGGG GGGGGGAPRG RYGPPSRRSE NRVVVSGLPP SGSWQDLKDH MREAGDVCYA DVYRDGTGVV EFVRKEDMTY AVRKLDNTKF RSHEGETAYI RVKVDGPRSP SYGRSRSRSR SRSRSRSRSN SRSRSYSPRR SRGSPRYSPR HSRSRSRT |
Serine/arginine-rich splicing factor 1 (SFRS1) belongs to the arginine/serine-rich splicing factor protein family, and functions in both constitutive and alternative pre-mRNA splicing. SFRS1 binds to pre-mRNA transcripts and components of the spliceosome, and can either initiate or inhibit splicing depending on the position of the pre-mRNA binding site. The ability of SFRS1 to activate splicing is controlled by phosphorylation and interactions with other splicing factor associated proteins.
SRSF1 Human Recombinant produced in E.coli is a single, non-glycosylated polypeptide chain containing 268 amino acids (1-248) and having a molecular mass of 29.9 kDa.SRSF1 is fused to a 20 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
| UniProt Protein Function: | SF2: a serine-arginine-rich splicing regulatory protein. Plays a role in preventing exon skipping, ensuring the accuracy of splicing and regulating alternative splicing. Interacts with other spliceosomal components, via the RS domains, to form a bridge between the 5' and 3' splice site binding components, U1 snRNP and U2AF. Can stimulate binding of U1 snRNP to a 5'-splice-site-containing pre-mRNA. Extensively phosphorylated on serine residues in the serine-arginine rich region. Three splice variant isoforms have been described. ASF/SF2 splicing enhancer (ASE) which can specifically activate ASE-dependent splicing. Isoform ASF-2 and isoform ASF-3 act as splicing repressors. |
| UniProt Protein Details: | Protein type:RNA-binding; RNA splicing; Spliceosome Chromosomal Location of Human Ortholog: 17q22 Cellular Component: nucleoplasm; cytoplasm; nuclear speck; nucleus Molecular Function:mRNA binding; protein binding; RS domain binding; RNA binding; nucleotide binding Biological Process: transcription from RNA polymerase II promoter; in utero embryonic development; RNA splicing; regulation of mRNA stability; regulation of translation; nuclear mRNA splicing, via spliceosome; nuclear mRNA 5'-splice site recognition; mRNA splice site selection; regulation of nuclear mRNA splicing, via spliceosome; mRNA export from nucleus; regulation of transcription, DNA-dependent; gene expression; mRNA 3'-end processing; mRNA processing; termination of RNA polymerase II transcription; cardiac muscle contraction |
| NCBI Summary: | This gene encodes a member of the arginine/serine-rich splicing factor protein family. The encoded protein can either activate or repress splicing, depending on its phosphorylation state and its interaction partners. Multiple transcript variants have been found for this gene. There is a pseudogene of this gene on chromosome 13. [provided by RefSeq, Jun 2014] |
| UniProt Code: | Q07955 |
| NCBI GenInfo Identifier: | 730773 |
| NCBI Gene ID: | 6426 |
| NCBI Accession: | Q07955.2 |
| UniProt Secondary Accession: | Q07955,Q13809, B2R6Z7, D3DTZ3, |
| UniProt Related Accession: | Q07955 |
| Molecular Weight: | Observed MW: 30kDaCalculated MW: 22kDa/27kDa/31kDa |
| NCBI Full Name: | Serine/arginine-rich splicing factor 1 |
| NCBI Synonym Full Names: | serine/arginine-rich splicing factor 1 |
| NCBI Official Symbol: | SRSF1�� |
| NCBI Official Synonym Symbols: | ASF; SF2; SFRS1; SF2p33; SRp30a�� |
| NCBI Protein Information: | serine/arginine-rich splicing factor 1; splicing factor 2; SR splicing factor 1; alternative-splicing factor 1; pre-mRNA-splicing factor SF2, P33 subunit; splicing factor, arginine/serine-rich, 30-KD, A |
| UniProt Protein Name: | Serine/arginine-rich splicing factor 1 |
| UniProt Synonym Protein Names: | Alternative-splicing factor 1; ASF-1; Splicing factor, arginine/serine-rich 1; pre-mRNA-splicing factor SF2, P33 subunit |
| UniProt Gene Name: | SRSF1�� |
| UniProt Entry Name: | SRSF1_HUMAN |
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