Human RUNX3 Recombinant Protein (RPPB4538)
- SKU:
- RPPB4538
- Product Type:
- Recombinant Protein
- Species:
- Human
- Uniprot:
- Q13761
Description
| Product Name: | Human RUNX3 Recombinant Protein |
| Product Code: | RPPB4538 |
| Size: | 10µg |
| Species: | Human |
| Target: | RUNX3 |
| Synonyms: | AML2, CBFA3, PEBP2aC, PEBP2A3, FLJ34510, MGC16070, RUNX3, Oncogene AML-2, Runt-related transcription factor 3, Core-binding factor subunit alpha-3, Acute myeloid leukemia 2 protein, Polyomavirus enhancer-binding protein 2 alpha C subunit, SL3-3 enhancer factor 1 alpha C subunit, SL3/AKV core-binding factor alpha C subunit. |
| Source: | Escherichia Coli |
| Physical Appearance: | Sterile filtered colorless solution. |
| Formulation: | RUNX3 Human solution (0.5mg/ml) containing 20mM Tris-HCl buffer (pH8.0), 20% glycerol, 0.1M NaCl and 1mM DTT. |
| Stability: | Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles. |
| Purity: | Greater than 95.0% as determined by SDS-PAGE. |
| Amino Acid Sequence: | MGSSHHHHHH SSGLVPRGSH MRSMVDVLAD HAGELVRTDS PNFLCSVLPS HWRCNKTLPV AFKVVALGDV PDGTVVTVMA GNDENYSAEL RNASAVMKNQ VARFNDLRFV GRSGRGKSFT LTITVFTNPT QVATYHRAIK VTVDGPREPR RHRQK |
RUNX3 is part of the RUNX family that mediates the expression of genes which participate in cellular differentiation and cell cycle progression. RUNX3 heterodimer and the beta subunit form a complex that binds to the core DNA sequence 5''-PYGPYGGT-3'' found in several enhancers and promoters, and can either activate or suppress transcription. RUNX3 interacts with additional transcription factors. RUNX3 is a good candidate for gastric cancer tumor suppressor diagnosis.
RUNX3 Recombinant Human produced in E.Coli is a single, non-glycosylated polypeptide chain containing 155 amino acids (53-186 a.a.) and having a molecular mass of 17.1 kDa. The RUNX3 is fused to 21 amino acid His-Tag at N-terminus and purified by proprietary chromatographic techniques.
| UniProt Protein Function: | AML2: CBF binds to the core site, 5'-PYGPYGGT-3', of a number of enhancers and promoters, including murine leukemia virus, polyomavirus enhancer, T-cell receptor enhancers, lck, IL-3 and GM-CSF promoters. Heterodimer of an alpha and a beta subunit. The alpha subunit binds DNA as a monomer and through the Runt domain. DNA- binding is increased by heterodimerization. Interacts with TLE1 and SUV39H1. The tyrosine phosphorylated form (via runt domain) interacts with SRC (via protein kinase domain). Interacts with FYN and LCK. 2 isoforms of the human protein are produced by alternative splicing. |
| UniProt Protein Details: | Protein type:DNA-binding; Transcription factor Chromosomal Location of Human Ortholog: 1p36 Cellular Component: cytoplasm; nuclear chromatin; nucleus Molecular Function:protein binding; transcription factor activity Biological Process: chondrocyte differentiation; hemopoiesis; negative regulation of cell cycle; negative regulation of epithelial cell proliferation; negative regulation of transcription from RNA polymerase II promoter; ossification; peripheral nervous system neuron development; positive regulation of transcription, DNA-dependent; protein amino acid phosphorylation; regulation of cell differentiation; regulation of transcription from RNA polymerase II promoter; transcription from RNA polymerase II promoter |
| NCBI Summary: | This gene encodes a member of the runt domain-containing family of transcription factors. A heterodimer of this protein and a beta subunit forms a complex that binds to the core DNA sequence 5'-PYGPYGGT-3' found in a number of enhancers and promoters, and can either activate or suppress transcription. It also interacts with other transcription factors. It functions as a tumor suppressor, and the gene is frequently deleted or transcriptionally silenced in cancer. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Mar 2016] |
| UniProt Code: | Q13761 |
| NCBI GenInfo Identifier: | 17368453 |
| NCBI Gene ID: | 864 |
| NCBI Accession: | Q13761.2 |
| UniProt Secondary Accession: | Q13761,Q12969, Q13760, B1AJV5, |
| UniProt Related Accession: | Q13761 |
| Molecular Weight: | 45,922 Da |
| NCBI Full Name: | Runt-related transcription factor 3 |
| NCBI Synonym Full Names: | runt related transcription factor 3 |
| NCBI Official Symbol: | RUNX3�� |
| NCBI Official Synonym Symbols: | AML2; CBFA3; PEBP2aC�� |
| NCBI Protein Information: | runt-related transcription factor 3 |
| UniProt Protein Name: | Runt-related transcription factor 3 |
| UniProt Synonym Protein Names: | Acute myeloid leukemia 2 protein; Core-binding factor subunit alpha-3; CBF-alpha-3; Oncogene AML-2; Polyomavirus enhancer-binding protein 2 alpha C subunit; PEA2-alpha C; PEBP2-alpha C; SL3-3 enhancer factor 1 alpha C subunit; SL3/AKV core-binding factor alpha C subunit |
| Protein Family: | Runt-related transcription factor |
| UniProt Gene Name: | RUNX3�� |
| UniProt Entry Name: | RUNX3_HUMAN |
