Human RHOG Recombinant Protein (RPPB4462)
- SKU:
- RPPB4462
- Product Type:
- Recombinant Protein
- Species:
- Human
- Uniprot:
- P84095
Description
| Product Name: | Human RHOG Recombinant Protein |
| Product Code: | RPPB4462 |
| Size: | 20µg |
| Species: | Human |
| Target: | RHOG |
| Synonyms: | Ras homolog family member G(rho G), rho-related GTP-binding protein RhoG, MGC125836, RhoG, ARHG, MGC125835. |
| Source: | Escherichia Coli |
| Physical Appearance: | Sterile Filtered colorless solution. |
| Formulation: | The RHOG solution (0.25mg/ml) contains 20mM Tris-HCl buffer (pH 8.0), 0.5M NaCl, 50mM Imidazole and 20% glycerol. |
| Stability: | Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles. |
| Purity: | Greater than 90% as determined by SDS-PAGE. |
| Amino Acid Sequence: | MRGSHHHHHH GMASMTGGQQ MGRDLYDDDD KDRWGSHMQS IKCVVVGDGA VGKTCLLICY TTNAFPKEYI PTVFDNYSAQ SAVDGRTVNL NLWDTAGQEE YDRLRTLSYP QTNVFVICFS IASPPSYENV RHKWHPEVCH HCPDVPILLV GTKKDLRAQP DTLRRLKEQG QAPITPQQGQ ALAKQIHAVR YLECSALQQD GVKEVFAEAV RAVLNPTPIK RGRSC |
RHOG belongs to the Rac subfamily of the Rho family of small G proteins. RHOG is a small monomeric GTP-binding protein (G protein), and is a key element of several intracellular signalling pathways. RHOG is needed for the formation of membrane ruffles during macropinocytosis. RHOG takes part in cell migration and is essential for the formation of cup-like structures during trans-endothelial migration of leukocytes. Similar to various small G proteins RHOG has a role in several cellular signalling mechanisms such as cell motility, gene transcription, endocytosis, neurite outgrowth, protection from anoikis and regulation of the neutrophil NADPH oxidase.
RHOG Human Recombinant produced in E. coli is a single polypeptide chain containing 225 amino acids (1-188) and having a molecular mass of 25.2 kDa. RHOG is fused to a 37 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
| UniProt Protein Function: | RHOG: Required for the formation of membrane ruffles during macropinocytosis. Plays a role in cell migration and is required for the formation of cup-like structures during trans-endothelial migration of leukocytes. In case of Salmonella enterica infection, activated by SopB and ARHGEF26/SGEF, which induces cytoskeleton rearrangements and promotes bacterial entry. Belongs to the small GTPase superfamily. Rho family. |
| UniProt Protein Details: | Protein type:G protein, monomeric, Rho; G protein, monomeric; Motility/polarity/chemotaxis Chromosomal Location of Human Ortholog: 11p15.5-p15.4 Cellular Component: cytosol; endoplasmic reticulum membrane; focal adhesion; plasma membrane Molecular Function:GTPase activity; protein binding Biological Process: actin cytoskeleton organization and biogenesis; platelet activation; positive regulation of cell proliferation; positive regulation of transcription, DNA-dependent; Rac protein signal transduction; regulation of small GTPase mediated signal transduction; Rho protein signal transduction |
| NCBI Summary: | This gene encodes a member of the Rho family of small GTPases, which cycle between inactive GDP-bound and active GTP-bound states and function as molecular switches in signal transduction cascades. Rho proteins promote reorganization of the actin cytoskeleton and regulate cell shape, attachment, and motility. The encoded protein facilitates translocation of a functional guanine nucleotide exchange factor (GEF) complex from the cytoplasm to the plasma membrane where ras-related C3 botulinum toxin substrate 1 is activated to promote lamellipodium formation and cell migration. Two related pseudogene have been identified on chromosomes 20 and X. [provided by RefSeq, Aug 2011] |
| UniProt Code: | P84095 |
| NCBI GenInfo Identifier: | 51338611 |
| NCBI Gene ID: | 391 |
| NCBI Accession: | P84095.1 |
| UniProt Secondary Accession: | P84095,P35238, Q8NI04, |
| UniProt Related Accession: | P84095 |
| Molecular Weight: | 21,309 Da |
| NCBI Full Name: | Rho-related GTP-binding protein RhoG |
| NCBI Synonym Full Names: | ras homolog family member G |
| NCBI Official Symbol: | RHOG�� |
| NCBI Official Synonym Symbols: | ARHG�� |
| NCBI Protein Information: | rho-related GTP-binding protein RhoG |
| UniProt Protein Name: | Rho-related GTP-binding protein RhoG |
| Protein Family: | Rho-related GTP-binding protein |
| UniProt Gene Name: | RHOG�� |
| UniProt Entry Name: | RHOG_HUMAN |
