Human RHOB Recombinant Protein (RPPB4459)
- SKU:
- RPPB4459
- Product Type:
- Recombinant Protein
- Species:
- Human
- Uniprot:
- P62745
Description
| Product Name: | Human RHOB Recombinant Protein |
| Product Code: | RPPB4459 |
| Size: | 10µg |
| Species: | Human |
| Target: | RHOB |
| Synonyms: | Rho-related GTP-binding protein RhoB, Rho cDNA clone 6, h6, RHOB, ARH6, ARHB, RHOH6, MST081, MSTP081. |
| Source: | Escherichia Coli |
| Physical Appearance: | Sterile Filtered colorless solution. |
| Formulation: | RHOB protein solution (0.25mg/ml) containing 20mM Tris-HCl buffer (pH 8.0), 2mM DTT, 20% glycerol and 0.2mM NaCl. |
| Stability: | Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles. |
| Purity: | Greater than 90.0% as determined by SDS-PAGE. |
| Amino Acid Sequence: | MGSSHHHHHH SSGLVPRGSH MAAIRKKLVV VGDGACGKTC LLIVFSKDEF PEVYVPTVFE NYVADIEVDG KQVELALWDT AGQEDYDRLR PLSYPDTDVI LMCFSVDSPD SLENIPEKWV PEVKHFCPNV PIILVANKKD LRSDEHVRTE LARMKQEPVR TDDGRAMAVR IQAYDYLECS AKTKEGVREV FETATRAALQ KRYGSQNGCI NCC |
Rho-related GTP-binding protein RhoB (RhoB) belongs to the Rho GTP-binding protein family. RhoB facilitates apoptosis in neoplastically transformed cells after DNA damage. RhoB has a negative role in tumorigenesis since deletion causes tumor formation.
RHOB produced in E.Coli is a single, non-glycosylated polypeptide chain containing 213 amino acids (1-193 a.a.) and having a molecular mass of 23.9kDa (Molecular weight on SDS-PAGE will appear higher).RHOB is fused to a 20 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
| UniProt Protein Function: | RHOB: a small G protein of the Rho family. Regulates a signal transduction pathway linking plasma membrane receptors to the assembly of focal adhesions and actin stress fibers. Controls the reorganization of actins into podosomes. Serves as a target for the yopT cysteine peptidase from Yersinia pestis, vector of the plague, and Yersinia pseudotuberculosis, which causes gastrointestinal disorders. |
| UniProt Protein Details: | Protein type:G protein; G protein, monomeric; G protein, monomeric, Rho; Motility/polarity/chemotaxis; Tumor suppressor Chromosomal Location of Human Ortholog: 2p24.1 Cellular Component: cleavage furrow; cytosol; endosome membrane; focal adhesion; nucleus; plasma membrane Molecular Function:GTP binding; GTPase activity; protein binding Biological Process: apoptosis; cell adhesion; cytokinesis; endosome to lysosome transport; negative regulation of cell cycle; negative regulation of cell migration; platelet activation; positive regulation of angiogenesis; positive regulation of apoptosis; regulation of cell migration; regulation of small GTPase mediated signal transduction; Rho protein signal transduction |
| UniProt Code: | P62745 |
| NCBI GenInfo Identifier: | 51338601 |
| NCBI Gene ID: | 388 |
| NCBI Accession: | P62745.1 |
| UniProt Secondary Accession: | P62745,P01121, Q5U0H6, Q7RTN5, Q7RTR9, Q9CUV7, B2R692 |
| UniProt Related Accession: | P62745 |
| Molecular Weight: | 22kDa |
| NCBI Full Name: | Rho-related GTP-binding protein RhoB |
| NCBI Synonym Full Names: | ras homolog family member B |
| NCBI Official Symbol: | RHOB�� |
| NCBI Official Synonym Symbols: | ARH6; ARHB; RHOH6; MST081; MSTP081�� |
| NCBI Protein Information: | rho-related GTP-binding protein RhoB |
| UniProt Protein Name: | Rho-related GTP-binding protein RhoB |
| UniProt Synonym Protein Names: | Rho cDNA clone 6; h6 |
| Protein Family: | Rho-related GTP-binding protein |
| UniProt Gene Name: | RHOB�� |
