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Human Protein Kinase R (PKR) ELISA Kit

SKU:
HUDL02314
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
P19525
Sensitivity:
0.055ng/mL
Range:
0.156-10ng/mL
ELISA Type:
Sandwich
Synonyms:
PRKR,PRK-R,EIF2AK2,EIF2AK1,Eukaryotic Translation Initiation Factor 2 Alpha Kinase 2,Interferon-induced, double-stranded RNA-activated protein kinase,P1/eIF-2A kinase
Reactivity:
Human
Sample Type:
Tissue Homogenates, Cell Lysates And Other Biological Fluids
$779
Frequently bought together:

Description

system_update_altDatasheet

Human Protein Kinase R (PKR) ELISA Kit

The Human Protein Kinase R (PKR) ELISA Kit is specifically designed for the precise quantification of protein kinase R levels in human samples such as serum, plasma, and cell culture supernatants. With its exceptional sensitivity and specificity, this kit ensures accurate and consistent results, making it a valuable tool for a variety of research applications.Protein kinase R is a vital enzyme involved in cellular response to stress, viral infection, and inflammation. Dysregulation of this protein has been linked to numerous diseases, including cancer, viral infections, and autoimmune disorders, highlighting its importance as a potential therapeutic target and biomarker for disease progression.

By utilizing the Human Protein Kinase R (PKR) ELISA Kit, researchers can gain valuable insights into the role of this protein in various physiological and pathological processes, thereby advancing our understanding of disease mechanisms and facilitating the development of novel therapeutic interventions.

Product Name:Human Protein Kinase R (PKR) ELISA Kit
Product Code:HUDL02314
Size:96 Assays
Target:PKR
Synonyms:PRKR, PRK-R, EIF2AK2, EIF2AK1, Eukaryotic Translation Initiation Factor 2 Alpha Kinase 2, Interferon-induced, double-stranded RNA-activated protein kinase, P1/eIF-2A kinase
Detection Method:Sandwich
Range:0.156-10ng/mL
Shelf Life:12 months
Note:For research use only
Recovery:Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
MatrixRecovery range (%)Average(%)
Serum (n=5)80-10291
EDTA plasma (n=5)81-9990
Heparin plasma (n=5)80-8984
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
Serum (n=5) 82-96% 83-98% 81-99% 93-101%
EDTA plasma (n=5) 88-101% 86-95% 90-102% 80-93%
Heparin plasma (n=5) 80-91% 82-90% 95-104% 79-95%
Intra-assay Precision:Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
StepProtocol
1. Prepare all reagents, samples and standards
2.Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.Aspirate and wash 3 times
5.Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.Aspirate and wash 5 times
7.Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.Add 50µL Stop Solution. Read at 450nm immediately.

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