The Human Plastin-3 (PLS3) ELISA Kit is specifically designed for the accurate measurement of plastin-3 levels in human samples such as serum, plasma, and cell culture supernatants. This ELISA kit boasts high sensitivity and specificity, ensuring reliable and reproducible results for a variety of research applications.Plastin-3, also known as T-plastin, is a crucial actin-binding protein that plays a key role in cell motility and cytoskeletal organization. It is implicated in various cellular processes such as cell migration, adhesion, and invasion, making it an important biomarker for studying cancer, autoimmune diseases, and inflammatory disorders.
With its high-quality components and user-friendly protocol, the Human Plastin-3 (PLS3) ELISA Kit is a valuable tool for researchers looking to explore the function and regulation of plastin-3 in different biological contexts. Discover new insights into cell biology and disease mechanisms with this innovative ELISA kit from Assay Genie.
Product Name:
Human Plastin-3 (PLS3) ELISA Kit
Product Code:
HUDL02349
Size:
96 Assays
Target:
PLS3
Synonyms:
T-plastin
Detection Method:
Competition
Range:
0.625-40ng/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
80-102
91
EDTA plasma (n=5)
81-100
90
Heparin plasma (n=5)
80-89
84
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. Note:To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity, and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 50µL standard or sample to each well.    And then add 50µL prepared Detection Reagent A immediately.    Shake and mix. Incubate 1 hour at 37°C
3.
Aspirate and wash 3 times
4.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
5.
Aspirate and wash 5 times
6.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
7.
Add 50µL Stop Solution. Read at 450 nm immediately.
