Human PINX1 Recombinant Protein (RPPB4251)
- SKU:
- RPPB4251
- Product Type:
- Recombinant Protein
- Species:
- Human
- Uniprot:
- Q96BK5
Description
| Product Name: | Human PINX1 Recombinant Protein |
| Product Code: | RPPB4251 |
| Size: | 20µg |
| Species: | Human |
| Target: | PINX1 |
| Synonyms: | PINX1, LPTL, LPTS, MGC8850, FLJ20565, Pin2-interacting protein X1, TRF1-interacting protein 1, Liver-related putative tumor suppressor, Protein 67-11-3. |
| Source: | Escherichia Coli |
| Physical Appearance: | Sterile filtered colorless solution. |
| Formulation: | The PINX1 protein contains 20mM Tris-HCl buffer pH-8, 1mM DTT and 10% glycerol. |
| Stability: | Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Avoid multiple freeze-thaw cycles. |
| Purity: | Greater than 95.0% as determined by analysis by SDS-PAGE. |
| Amino Acid Sequence: | MGSSHHHHHH SSGLVPRGSH MSMLAERRRK QKWAVDPQNT AWSNDDSKFG QRMLEKMGWS KGKGLGAQEH GATDHIKVQV KNNHLGLGAT INNEDNWIAH QDDFNQLLAE LNTCHGQETT DSSDKKEKKS FSLEEKSKIS KNRVHYMKFT KGKDLSSRSK TDLDCIFGKR QSKKTPEGDA SPSTPEENET TTTSAFTIQE YFAKRMAALK NKPQVPVPGS DISETQVERK RGKKINKEAT GKDVESYLQP KAKRHTEGKP ERAEAQERVA KKKSAPAEEQ LRGPCWDQSS KASAQDAGDH VQPPEGRDFT LKPKKRRGKK KLQKPVEIAE DATLEETLVK KKKKKDSK |
PINX1 is a common expressed protein that localizes to nucleoli and telomere speckles. PINX1 contains a Telomerase Inhibiting Domain that is caable of binding MCRS1, TERT and TERF1. PINX1 has been shown to be a potent telomerase inhibitor and putative tumor suppressor. PINX1 is recruited to chromosome periphery by Nucleolin, their complex is necessary for faithful chromosome congression. PINX1 regulates the nucleolar accumulation and telomeric association of TRF1. PINX1 is involved in gastric cancer development. PINX1 expression is a sign of gastric cancer development. Constitutive expression of PINX1 attributes to telomere maintenance by telomerase and tumorigenicity in cancer cells.
Recombinant Human PINX1 produced in E.Coli is a single, non-glycosylated polypeptide chain containing 348 amino acids (1-328 a.a) and having a molecular mass of 39.1 kDa. PINX1 is fused to 20 amino acid His Tag at N-terminus and purified by proprietary chromatographic techniques.
| UniProt Protein Function: | PINX1: Microtubule-binding protein essential for faithful chromosome segregation. Mediates TRF1 and TERT accumulation in nucleolus and enhances TRF1 binding to telomeres. Inhibits telomerase activity. May inhibit cell proliferation and act as tumor suppressor. Belongs to the PINX1 family. 2 isoforms of the human protein are produced by alternative splicing. |
| UniProt Protein Details: | Protein type:Nucleolus; Tumor suppressor; Inhibitor Chromosomal Location of Human Ortholog: 8p23 Cellular Component: cytoplasm; intracellular membrane-bound organelle; kinetochore; mitochondrion; nuclear chromosome; nuclear chromosome, telomeric region; nucleolus; nucleus; spindle Molecular Function:protein binding Biological Process: mitotic metaphase plate congression; negative regulation of cell proliferation; negative regulation of protein ubiquitination; negative regulation of telomerase activity; negative regulation of telomere maintenance via telomerase; regulation of protein stability; regulation of telomerase activity; telomere maintenance via telomerase |
| UniProt Code: | Q96BK5 |
| NCBI GenInfo Identifier: | 547234890 |
| NCBI Gene ID: | 54984 |
| NCBI Accession: | NP_001271285.1 |
| UniProt Secondary Accession: | Q96BK5,Q548A5, Q6QWG9, Q7Z7J8, Q96QD7, Q9HBU7, Q9NWW2 B2R9B1, |
| UniProt Related Accession: | Q96BK5 |
| Molecular Weight: | 19,714 Da |
| NCBI Full Name: | PIN2/TERF1-interacting telomerase inhibitor 1 isoform 2 |
| NCBI Synonym Full Names: | PIN2/TERF1 interacting, telomerase inhibitor 1 |
| NCBI Official Symbol: | PINX1�� |
| NCBI Official Synonym Symbols: | LPTL; LPTS�� |
| NCBI Protein Information: | PIN2/TERF1-interacting telomerase inhibitor 1 |
| UniProt Protein Name: | PIN2/TERF1-interacting telomerase inhibitor 1 |
| UniProt Synonym Protein Names: | Liver-related putative tumor suppressor; Pin2-interacting protein X1; Protein 67-11-3; TRF1-interacting protein 1 |
| Protein Family: | PIN2/TERF1-interacting telomerase inhibitor |
| UniProt Gene Name: | PINX1�� |
| UniProt Entry Name: | PINX1_HUMAN |
