The Human PGC (Pepsinogen C) ELISA Kit is a specialized assay designed for the quantitative assessment of pepsinogen C levels in human samples. Pepsinogen C, also known as gastricsin or progastricsin, is an enzyme precursor secreted by the gastric chief cells in the stomach mucosa. It serves as a valuable biomarker for evaluating gastric mucosal health and assessing the functionality of the stomach's chief cells.
This ELISA kit enables precise measurement of pepsinogen C concentrations, offering insights into gastric function, mucosal integrity, and chief cell activity. Pepsinogen C levels can provide valuable information about gastric health and physiology, allowing researchers and clinicians to monitor conditions such as gastritis, peptic ulcers, and gastric cancer with greater accuracy and reliability. The Human PGC ELISA Kit ensures exceptional sensitivity and specificity, facilitating reliable and reproducible results. Manufactured under rigorous quality control standards, this kit delivers robust performance and user-friendly operation, making it a preferred tool for research, clinical diagnostics, and studies focusing on gastric health and associated conditions.
Product Name:
Human PGC (Pepsinogen C) ELISA Kit
SKU:
AEES00658
Target:
Human PGC (Pepsinogen C)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
0.28 ng/mL
Detection range:
0.47-30 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human PGC. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human PGC and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human PGC, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human PGC. You can calculate the concentration of Human PGC in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
88-100
94-108
99-111
Average (%)
94
100
106
1:4
Range (%)
86-99
91-103
95-111
Average (%)
91
97
103
1:8
Range (%)
90-103
97-110
96-113
Average (%)
96
103
104
1:16
Range (%)
84-95
93-107
97-111
Average (%)
90
101
103
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
97-107
102
EDTA plasma (n=5)
93-107
98
Cell culture media (n=5)
85-100
92
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
1.44
3.35
13.73
1.38
3.17
13.54
Standard deviation
0.08
0.17
0.46
0.07
0.13
0.48
C V (%)
5.23
5.18
3.34
5.16
4.19
3.51
Sample type &Sample volume:
; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Human PGC concentrations in .
Specificity:
This kit recognizes Human PGC in samples. No significant cross-reactivity or interference between Human PGC and analogues was observed.
