The Human NRG-1 (Neuregulin 1) ELISA Kit is meticulously crafted to enable the precise quantification of Neuregulin 1 levels in diverse human biological samples. Neuregulin 1, a critical growth factor known for its multifaceted roles in cell signaling, development, and tissue regeneration, plays a pivotal role in various physiological processes, including neural development and cardiac function.
Accurate measurement of Neuregulin 1 is vital for unraveling its intricate functions in health and disease, particularly in neurobiology and cardiovascular research. Our NRG-1 ELISA Kit guarantees exceptional sensitivity and specificity, ensuring the delivery of reliable and reproducible results. Engineered under rigorous quality standards, this kit ensures robust performance and seamless usability, rendering it an ideal choice for both rigorous research investigations and clinical applications. Rely on our NRG-1 ELISA Kit to achieve precise and trustworthy quantification of this pivotal biomarker in your scientific inquiries.
Product Name:
Human NRG-1 (Neuregulin 1) ELISA Kit
SKU:
AEES00126
Target:
Human NRG-1 (Neuregulin 1)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
9.38 pg/mL
Detection range:
15.63-1000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human NRG-1. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human NRG-1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human NRG-1, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human NRG-1. You can calculate the concentration of Human NRG-1 in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
93-108
93-106
97-108
Average (%)
100
98
102
1:4
Range (%)
91-101
87-97
91-103
Average (%)
96
91
96
1:8
Range (%)
94-106
83-94
88-101
Average (%)
101
91
95
1:16
Range (%)
89-99
94-106
89-102
Average (%)
95
98
95
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
85-97
90
EDTA plasma (n=5)
97-110
104
Cell culture media (n=5)
92-104
99
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
49.09
112.92
495.58
45.27
108.53
485.65
Standard deviation
2.76
5.33
23.79
2.72
5.21
21.32
C V (%)
5.62
4.72
4.8
6.01
4.8
4.39
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Human NRG-1 concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human NRG-1 in samples. No significant cross-reactivity or interference between Human NRG-1 and analogues was observed.
