Human Neutrophil Cytosolic Factor 1 (NCF1) ELISA Kit
The Human Neutrophil Cytosolic Factor 1 (NCF1) ELISA Kit is specifically designed for the precise measurement of NCF1 levels in human samples including serum, plasma, and cell culture supernatants. With its high sensitivity and specificity, this kit provides reliable and reproducible results, making it an indispensable tool for various research applications. NCF1 is a key component in the regulation of neutrophil function and immune responses. Dysfunction of NCF1 has been associated with autoimmune diseases, inflammatory conditions, and immune deficiencies.
Understanding the levels of NCF1 in biological samples can provide valuable insights into the immune system and pave the way for potential diagnostic and therapeutic strategies. Researchers and clinicians alike can benefit from the accurate quantification of NCF1 using this ELISA kit, allowing for in-depth studies in immunology, inflammation, and autoimmune disorders. Trust in the Human Neutrophil Cytosolic Factor 1 (NCF1) ELISA Kit for precise and reliable results in your research endeavors.
Product Name:
Human Neutrophil Cytosolic Factor 1 (NCF1) ELISA Kit
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
80-102
91
EDTA plasma (n=5)
81-100
90
Heparin plasma (n=5)
80-89
84
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
