Human MLF1 Recombinant Protein (RPPB3979)
- SKU:
- RPPB3979
- Product Type:
- Recombinant Protein
- Species:
- Human
- Uniprot:
- P58340
Description
| Product Name: | Human MLF1 Recombinant Protein |
| Product Code: | RPPB3979 |
| Size: | 20µg |
| Species: | Human |
| Target: | MLF1 |
| Synonyms: | Myeloid leukemia factor 1, Myelodysplasia-myeloid leukemia factor 1, MLF1. |
| Source: | Escherichia Coli |
| Physical Appearance: | Sterile Filtered colorless solution. |
| Formulation: | The MLF1 solution (0.5 mg/ml) contains 20mM Tris-HCl buffer (pH8.0), 40% glycerol, 5mM DTT and 200mM NaCl. |
| Stability: | Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles. |
| Purity: | Greater than 85.0% as determined by SDS-PAGE. |
| Amino Acid Sequence: | MGSSHHHHHH SSGLVPRGSH MFRMLNSSFE DDPFFSESIL AHRENMRQMI RSFSEPFGRD LLSISDGRGR AHNRRGHNDG EDSLTHTDVS SFQTMDQMVS NMRNYMQKLE RNFGQLSVDP NGHSFCSSSV MTYSKIGDEP PKVFQASTQT RRAPGGIKET RKAMRDSDSG LEKMAIGHHI HDRAHVIKKS KNKKTGDEEV NQEFINMNES DAHAFDEEWQ SEVLKYKPGR HNLGNTRMRS VGHENPGSRE LKRREKPQQS PAIEHGRRSN VLGDKLHIKG SSVKSNKK |
Myeloid leukemia factor 1 (MLF1) is a member of the MLF family, and is a widely expressed negative regulator of cell cycle progression functioning upstream of the tumor suppressor p53. MLF1 hinders the erythropoietin-induced erythroid terminal differentiation by averting cells from exiting the cell cycle through suppression of CDKN1B/p27Kip1 levels. MLF1 generally functions in multi-potent progenitor cells, and its dysregulation may be to some extent responsible for leukemogenesis. Translocations between the MLF1 gene and nucleophosmin are linked to myelodysplastic syndrome and acute myeloid leukemia.
MLF1 Human Recombinant fused with a�20 amino acid His tag at N-terminus produced in E.Coli is a single, non-glycosylated, polypeptide chain containing�288 amino acids (1-268 a.a.) and having a molecular mass of 32.8kDa (Molecular weight on SDS-PAGE will appear higher). The MLF1 is purified by proprietary chromatographic techniques.
| UniProt Protein Function: | MLF1: Involved in lineage commitment of primary hemopoietic progenitors by restricting erythroid formation and enhancing myeloid formation. Interferes with erythropoietin-induced erythroid terminal differentiation by preventing cells from exiting the cell cycle through suppression of CDKN1B/p27Kip1 levels. Suppresses RFWD2/COP1 activity via CSN3 which activates p53 and induces cell cycle arrest. Binds DNA and affects the expression of a number of genes so may function as a transcription factor in the nucleus. A chromosomal aberration involving MLF1 is a cause of myelodysplastic syndrome (MDS). Translocation t(3;5)(q25.1;q34) with NPM1/NPM. Belongs to the MLF family. 5 isoforms of the human protein are produced by alternative splicing. |
| UniProt Protein Details: | Protein type:Cell cycle regulation Chromosomal Location of Human Ortholog: 3q25.1 Cellular Component: cytoplasm; nucleus Molecular Function:DNA binding; protein binding; protein domain specific binding Biological Process: cell cycle arrest; myeloid progenitor cell differentiation; transcription, DNA-dependent Disease: Leukemia, Acute Myeloid |
| NCBI Summary: | This gene encodes an oncoprotein which is thought to play a role in the phenotypic determination of hemopoetic cells. Translocations between this gene and nucleophosmin have been associated with myelodysplastic syndrome and acute myeloid leukemia. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2010] |
| UniProt Code: | P58340 |
| NCBI GenInfo Identifier: | 17368170 |
| NCBI Gene ID: | 4291 |
| NCBI Accession: | P58340.1 |
| UniProt Secondary Accession: | P58340,Q2TLE3, Q2TLE5, Q8N8F8, Q96MH1, E9PEU9, |
| UniProt Related Accession: | P58340 |
| Molecular Weight: | 29,095 Da |
| NCBI Full Name: | Myeloid leukemia factor 1 |
| NCBI Synonym Full Names: | myeloid leukemia factor 1 |
| NCBI Official Symbol: | MLF1�� |
| NCBI Protein Information: | myeloid leukemia factor 1 |
| UniProt Protein Name: | Myeloid leukemia factor 1 |
| UniProt Synonym Protein Names: | Myelodysplasia-myeloid leukemia factor 1 |
| Protein Family: | Myeloid leukemia factor |
| UniProt Gene Name: | MLF1�� |
| UniProt Entry Name: | MLF1_HUMAN |
