Human MBD3 Recombinant Protein (RPPB3935)
- SKU:
- RPPB3935
- Product Type:
- Recombinant Protein
- Species:
- Human
- Uniprot:
- O95983
Description
| Product Name: | Human MBD3 Recombinant Protein |
| Product Code: | RPPB3935 |
| Size: | 10µg |
| Species: | Human |
| Target: | MBD3 |
| Synonyms: | Methyl-CpG Binding Domain Protein 3, Methyl-CpG-Binding Domain Protein 3, Methyl-CpG-Binding Protein MBD3. |
| Source: | Escherichia Coli |
| Physical Appearance: | Sterile Filtered colorless solution. |
| Formulation: | The MBD3 solution (0.25mg/1ml) contains 20mM Tris-HCl buffer (pH 8.0), 0.2M NaCl, 1mM DTT, 1mM EDTA and 40% glycerol. |
| Stability: | Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles. |
| Purity: | Greater than 85% as determined by SDS-PAGE. |
| Amino Acid Sequence: | MGSSHHHHHH SSGLVPRGSH MGSMERKRWE CPALPQGWER EEVPRRSGLS AGHRDVFYYS PSGKKFRSKP QLARYLGGSM DLSTFDFRTG KMLMSKMNKS RQRVRYDSSN QVKGKPDLNT ALPVRQTASI FKQPVTKITN HPSNKVKSDP QKAVDQPRQL FWEKKLSGLN AFDIAEELVK TMDLPKGLQG VGPGCTDETL LSAIASALHT STMPITGQLS AAVEKNPGVW LNTTQPLCKA FMVTDEDIRK QEELVQQVRK RLEEALMADM LAHVEELARD GEAPLDKACA EDDDEEDEEE EEEEPDPDPE MEHV |
Methyl-CpG-binding domain protein 3 (MBD3), belongs to the MBD family of transcriptional repressors. Human proteins MECP2, MBD1, MBD2, MBD3, and MBD4 includes a family of nuclear proteins related by the presence of a methyl-CpG binding domain (MBD) in each protein. Nevertheless, not like the other family members, MBD3 is not capable of binding to methylated DNA. MBD3 is a subunit of the NuRD, a multisubunit complex having nucleosome remodeling and histone deacetylase activities. The predicted MBD3 protein shares 71% and 94% identity with MBD2 (isoform 1) and mouse Mbd3. MBD3 mediates the association of metastasis-associated protein 2 (MTA2) with the core histone deacetylase complex.
MBD3 Human Recombinant produced in E. coli is a single polypeptide chain containing 314 amino acids (1-291) and having a molecular mass of 35.2 kDa. MBD3 is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
| UniProt Protein Function: | MBD3: methyl-CpG binding (MBD) proteins appear to bind differentially methylated domains (DMDs) of DNA, to recruit repression complexes, and to silence the locus. The methyl CpG binding domain of human MBD3 may not directly bind mCpG but does interact with the NuRD/Mi2 components HDAC1 and MTA2. Plays a role in histone deacetylation and nucleosome remodeling. Increased levels have been reported in lung cancer and glioblastomas and may be a therapeutic target. |
| UniProt Protein Details: | Protein type:Gene silencing; Transcription, coactivator/corepressor Chromosomal Location of Human Ortholog: 19p13.3 Cellular Component: nuclear chromatin; nucleoplasm; protein complex Molecular Function:DNA binding; histone deacetylase activity; methyl-CpG binding; nucleosomal DNA binding; protein binding Biological Process: ATP-dependent chromatin remodeling; methylation-dependent chromatin silencing; negative regulation of transcription from RNA polymerase II promoter |
| NCBI Summary: | DNA methylation is the major modification of eukaryotic genomes and plays an essential role in mammalian development. This gene belongs to a family of nuclear proteins which are characterized by the presence of a methyl-CpG binding domain (MBD). The encoded protein is a subunit of the NuRD, a multisubunit complex containing nucleosome remodeling and histone deacetylase activities. Unlike the other family members, the encoded protein is not capable of binding to methylated DNA. The protein mediates the association of metastasis-associated protein 2 with the core histone deacetylase complex. Alternative splicing results in multiple transcript variants of this gene. [provided by RefSeq, Jul 2013] |
| UniProt Code: | O95983 |
| NCBI GenInfo Identifier: | 50400820 |
| NCBI Gene ID: | 53615 |
| NCBI Accession: | O95983.1 |
| UniProt Secondary Accession: | O95983,Q6PIL9, Q6PJZ9, Q86XF4, A8K4B7, D6W5Z2, |
| UniProt Related Accession: | O95983 |
| Molecular Weight: | 29,014 Da |
| NCBI Full Name: | Methyl-CpG-binding domain protein 3 |
| NCBI Synonym Full Names: | methyl-CpG binding domain protein 3 |
| NCBI Official Symbol: | MBD3�� |
| NCBI Protein Information: | methyl-CpG-binding domain protein 3 |
| UniProt Protein Name: | Methyl-CpG-binding domain protein 3 |
| UniProt Synonym Protein Names: | Methyl-CpG-binding protein MBD3 |
| Protein Family: | Methyl-CpG-binding domain protein |
| UniProt Gene Name: | MBD3�� |
| UniProt Entry Name: | MBD3_HUMAN |
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