Human KRT8 Recombinant Protein (RPPB3823)
- SKU:
- RPPB3823
- Product Type:
- Recombinant Protein
- Species:
- Human
- Uniprot:
- P05787
Description
| Product Name: | Human KRT8 Recombinant Protein |
| Product Code: | RPPB3823 |
| Size: | 20µg |
| Species: | Human |
| Target: | KRT8 |
| Synonyms: | Keratin type II cytoskeletal 8, Cytokeratin-8, CK-8, Keratin-8, K8, KRT8, CYK8, KO, CK8, K2C8, CARD2. |
| Source: | Escherichia Coli |
| Physical Appearance: | Sterile Filtered White lyophilized (freeze-dried) powder. |
| Formulation: | The protein (1mg/ml) was lyophilized after from a sterile solution containing 30mM Tris-HCl pH-8, 9.5M urea, 2mM DTT, 2mM EDTA and 10mM methylammonium chloride. |
| Solubility: | It is recommended to reconstitute the lyophilized KRT8 in sterile 18M?-cm H2O not less than 100�g/ml, which can then be further diluted to other aqueous solutions. |
| Stability: | Lyophilized KRT8 although stable at room temperature for 3 weeks, should be stored at 2-8°C. Upon reconstitution KRT8 should be stored at 4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).�Please prevent freeze-thaw cycles. |
| Purity: | Greater than 95.0% as determined by:(a) Analysis by RP-HPLC.(b) Analysis by SDS-PAGE. |
Keratin 8 and 18 (K8/18) are the major components of intermediate filament (IF) proteins of simple or single-layered epithelia.
Cytokeratin 8 Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain having a molecular mass of 53,532 Dalton. The KRT8 is purified by proprietary chromatographic techniques.
| UniProt Protein Function: | K8: a type II cytoskeletal keratin. The keratins are intermediate filament proteins responsible for the structural integrity of epithelial cells and are subdivided into cytokeratins and hair keratins. Phosphorylation of keratins at specific sites affects their organization, assembly dynamics, and their interaction with signaling molecules. Phsophorylated by p38 kinase, regulating cellular keratin filament reorganization. Phosphorylation on serine residues is enhanced during EGF stimulation and mitosis. Mutation of this protein is a risk factor for cryptogenic liver failure. |
| UniProt Protein Details: | Protein type:Cytoskeletal Chromosomal Location of Human Ortholog: 12q13 Cellular Component: dystrophin-associated glycoprotein complex; nucleoplasm; intermediate filament cytoskeleton; nuclear matrix; costamere; cytoplasm; keratin filament; intermediate filament; intercellular junction; Z disc; nucleus; sarcolemma Molecular Function:protein binding; protein complex binding; structural molecule activity Biological Process: tumor necrosis factor-mediated signaling pathway; viral reproduction; response to other organism; sarcomere organization; response to hydrostatic pressure Disease: Cirrhosis, Familial |
| NCBI Summary: | This gene is a member of the type II keratin family clustered on the long arm of chromosome 12. Type I and type II keratins heteropolymerize to form intermediate-sized filaments in the cytoplasm of epithelial cells. The product of this gene typically dimerizes with keratin 18 to form an intermediate filament in simple single-layered epithelial cells. This protein plays a role in maintaining cellular structural integrity and also functions in signal transduction and cellular differentiation. Mutations in this gene cause cryptogenic cirrhosis. Alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Jan 2012] |
| UniProt Code: | P05787 |
| NCBI GenInfo Identifier: | 90110027 |
| NCBI Gene ID: | 3856 |
| NCBI Accession: | P05787.7 |
| UniProt Secondary Accession: | P05787,Q14099, Q14716, Q14717, Q53GJ0, Q6DHW5, Q6GMY0 Q6P4C7, Q96J60, A8K4H3, B0AZN5, F8VXB4, |
| UniProt Related Accession: | P05787 |
| Molecular Weight: | 483 |
| NCBI Full Name: | Keratin, type II cytoskeletal 8 |
| NCBI Synonym Full Names: | keratin 8 |
| NCBI Official Symbol: | KRT8�� |
| NCBI Official Synonym Symbols: | K8; KO; CK8; CK-8; CYK8; K2C8; CARD2�� |
| NCBI Protein Information: | keratin, type II cytoskeletal 8; cytokeratin 8; cytokeratin-8; type-II keratin Kb8 |
| UniProt Protein Name: | Keratin, type II cytoskeletal 8 |
| UniProt Synonym Protein Names: | Cytokeratin-8; CK-8; Keratin-8; K8; Type-II keratin Kb8 |
| Protein Family: | Keratin |
| UniProt Gene Name: | KRT8�� |
| UniProt Entry Name: | K2C8_HUMAN |
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