The Human Fibrinogen Alpha (FGA) ELISA Kit is a cutting-edge tool for accurate detection of fibrinogen alpha levels in human serum, plasma, and cell culture supernatants. This kit offers high sensitivity and specificity, ensuring dependable and reproducible results for a wide range of research applications.Fibrinogen alpha is a vital component of the blood clotting process, playing a crucial role in wound healing and preventing excessive bleeding. Dysregulation of fibrinogen alpha has been linked to various conditions such as cardiovascular diseases, liver disease, and inflammatory disorders, making it a valuable biomarker for studying these diseases and potential treatment strategies.
The Human Fibrinogen Alpha (FGA) ELISA Kit is an indispensable tool for researchers and clinicians looking to explore the role of fibrinogen alpha in health and disease. With its unparalleled performance and ease of use, this kit is an essential addition to any laboratory focused on advancing our understanding of fibrinogen alpha biology and its clinical implications.
Product Name:
Human Fibrinogen Alpha (FGa) ELISA Kit
Product Code:
HUDL01048
Size:
96 Assays
Target:
FGa
Synonyms:
Fib2, FG-A, Fibrinopeptide A
Detection Method:
Sandwich
Range:
3.125-200ng/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
80-102
91
EDTA plasma (n=5)
81-99
90
Heparin plasma (n=5)
80-89
84
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
