Human FBLIM1 Recombinant Protein (RPPB3519)
- SKU:
- RPPB3519
- Product Type:
- Recombinant Protein
- Species:
- Human
- Uniprot:
- Q8WUP2
Description
| Product Name: | Human FBLIM1 Recombinant Protein |
| Product Code: | RPPB3519 |
| Size: | 10µg |
| Species: | Human |
| Target: | FBLIM1 |
| Synonyms: | Filamin binding LIM protein 1, CAL, FBLP-1, FBLP1, RP11-169K16.5, Migfilin, Mitogen-inducible 2-interacting protein, MIG2-interacting protein. |
| Source: | Escherichia Coli |
| Physical Appearance: | Sterile Filtered colorless solution. |
| Formulation: | The FBLIM1 solution (0.25mg/ml) contains 20mM Tris-HCl buffer (pH 8.0), 0.15M NaCl, 10% glycerol and 1mM DTT. |
| Stability: | Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles. |
| Purity: | Greater than 85.0% as determined by SDS-PAGE. |
| Amino Acid Sequence: | MGSSHHHHHH SSGLVPRGSH MGSMASKPEK RVASSVFITL APPRRDVAVA EEVRQAVCEA RRGRPWEAPA PMKTPEAGLA GRPSPWTTPG RAAATVPAAP MQLFNGGCPP PPPVLDGEDV LPDLDLLPPP PPPPPVLLPS EEEAPAPMGA SLIADLEQLH LSPPPPPPQA PAEGPSVQPG PLRPMEEELP PPPAEPVEKG ASTDICAFCH KTVSPRELAV EAMKRQYHAQ CFTCRTCRRQ LAGQSFYQKD GRPLCEPCYQ DTLERCGKCG EVVRDHIIRA LGQAFHPSCF TCVTCARCIG DESFALGSQN EVYCLDDFYR KFAPVCSICE NPIIPRDGKD AFKIECMGRN FHENCYRCED CRILLSVEPT DQGCYPLNNH LFCKPCHVKR SAAGCC |
�Filamin binding LIM protein 1 (FBLIM1) plays a role as an anchoring site for cell-ECM adhesion proteins and filamin-containing actin filaments. FBLIM1 is involved in cell shape spreading and motility. FBLIM1 participates in the regulation of filamin-mediated cross-linking and stabilization of actin filaments. FBLIM1 promotes stimulation of integrins and regulates integrin-mediated cell-cell adhesion.
FBLIM1 Human Recombinant produced in E.coli is a single, non-glycosylated polypeptide chain containing 396 amino acids (1-373) and having a molecular mass of 43.1 kDa. FBLIM1 is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
| UniProt Protein Function: | FBLIM1: Serves as an anchoring site for cell-ECM adhesion proteins and filamin-containing actin filaments. Is implicated in cell shape modulation (spreading) and motility. May participate in the regulation of filamin-mediated cross-linking and stabilization of actin filaments. May also regulate the assembly of filamin- containing signaling complexes that control actin assembly. Promotes dissociation of FLNA from ITGB3 and ITGB7. Promotes activation of integrins and regulates integrin-mediated cell-cell adhesion. 3 isoforms of the human protein are produced by alternative splicing. |
| UniProt Protein Details: | Protein type:Motility/polarity/chemotaxis Chromosomal Location of Human Ortholog: 1p36.21 Cellular Component: cytosol; focal adhesion; stress fiber Molecular Function:filamin binding; protein binding Biological Process: cell-cell adhesion; regulation of integrin activation |
| NCBI Summary: | This gene encodes a protein with an N-terminal filamin-binding domain, a central proline-rich domain, and, multiple C-terminal LIM domains. This protein localizes at cell junctions and may link cell adhesion structures to the actin cytoskeleton. This protein may be involved in the assembly and stabilization of actin-filaments and likely plays a role in modulating cell adhesion, cell morphology and cell motility. This protein also localizes to the nucleus and may affect cardiomyocyte differentiation after binding with the CSX/NKX2-5 transcription factor. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Jul 2008] |
| UniProt Code: | Q8WUP2 |
| NCBI GenInfo Identifier: | 125987829 |
| NCBI Gene ID: | 54751 |
| NCBI Accession: | Q8WUP2.2 |
| UniProt Secondary Accession: | Q8WUP2,Q5VVE0, Q5VVE1, Q8IX23, Q96T00, Q9UFD6, B3KNY0 |
| UniProt Related Accession: | Q8WUP2 |
| Molecular Weight: | 30,770 Da |
| NCBI Full Name: | Filamin-binding LIM protein 1 |
| NCBI Synonym Full Names: | filamin binding LIM protein 1 |
| NCBI Official Symbol: | FBLIM1�� |
| NCBI Official Synonym Symbols: | CAL; FBLP1; FBLP-1�� |
| NCBI Protein Information: | filamin-binding LIM protein 1 |
| UniProt Protein Name: | Filamin-binding LIM protein 1 |
| UniProt Synonym Protein Names: | Migfilin; Mitogen-inducible 2-interacting protein; MIG2-interacting protein |
| Protein Family: | Filamin-binding LIM protein |
| UniProt Gene Name: | FBLIM1�� |
| UniProt Entry Name: | FBLI1_HUMAN |
